黄单胞菌属植物病原菌株的高效转化

Murooka Y, Iwamoto H, Hamamoto A, Yamauchi T

J Bacteriol. 1987 Sep;169(9):4406-9. doi: 10.1128/jb.169.9.4406-4409.1987.

Efficient transformation and conjugation systems for use in phytopathogenic strains of Xanthomonas species were developed with a dual-function plasmid vector, pBXC12, which was constructed from a newly isolated Xanthomonas citri plasmid, pXCL6, and pBR328. By using this system, pBR328 could also transform the same strains of Xanthomonas. The systems make useful cloning vectors for the study of genes involved in the plant pathogenesis of this species.

利用一种双功能质粒载体pBXC12，开发了用于柑橘溃疡病菌种植物病原菌株的高效转化和结合系统。pBXC12由新分离的柑橘黄单胞菌质粒pXCL6和pBR328构建而成。通过使用该系统，pBR328也可以转化相同的柑橘溃疡病菌株。这些系统为研究该物种植物致病相关基因提供了有用的克隆载体。