岛状噬纤维菌 A16 对脱氧雪腐镰刀菌烯醇及其衍生物的生物降解作用。
Biodegradation of deoxynivalenol and its derivatives by Devosia insulae A16.
机构信息
Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences/Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base/Key Laboratory of Control Technology and Standard for Agro-Product Quality and Safety, Ministry of Agriculture/Key Laboratory of Agro-Product Safety Risk Evaluation, Ministry of Agriculture/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China.
Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences/Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base/Key Laboratory of Control Technology and Standard for Agro-Product Quality and Safety, Ministry of Agriculture/Key Laboratory of Agro-Product Safety Risk Evaluation, Ministry of Agriculture/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China.
出版信息
Food Chem. 2019 Mar 15;276:436-442. doi: 10.1016/j.foodchem.2018.10.011. Epub 2018 Oct 3.
Deoxynivalenol (DON), a notorious mycotoxin mainly found in Fusarium-contaminated crops, causes great loss in livestock farming and severe safety risks to human health. Here we report the isolation of a Gram-negative bacterial strain with effective biodegrading abilities on DON and its derivatives including 3-acetyl-DON and 15-acetyl-DON. The strain was identified as Devosia insulae A16 on the basis of morphological and physiological characteristics and 16S rRNA-based phylogenetic analysis. D. insulae A16 was able to degrade 88% of 20 mg/l DON within 48 h under aerobic conditions at 35 °C and neutral pH. The major degradation product of DON and its derivatives was 3-keto-DON by the oxidation of the hydroxyl group at C-3. Both 3-acetyl-DON and 15-acetyl-DON underwent a deacetylation reaction to generate DON prior to the degradation to 3-keto-DON. The results provide the potential use of D. insulae A16 as a biodegradation agent to control DON contamination in cereals.
脱氧雪腐镰刀菌烯醇（DON）是一种主要存在于镰刀菌污染作物中的著名霉菌毒素，给畜牧业造成了巨大损失，并对人类健康构成严重安全风险。在这里，我们报告了一株革兰氏阴性细菌的分离，该细菌对 DON 及其衍生物（包括 3-乙酰-DON 和 15-乙酰-DON）具有有效的生物降解能力。该菌株基于形态学和生理学特征以及基于 16S rRNA 的系统发育分析，被鉴定为岛状德沃斯氏菌 A16。在 35°C 和中性 pH 条件下，需氧条件下，D. insulae A16 能够在 48 小时内将 20mg/L 的 DON 降解 88%。DON 和其衍生物的主要降解产物是通过 C-3 羟基氧化生成的 3-酮-DON。3-乙酰-DON 和 15-乙酰-DON 都经历了脱乙酰化反应，在降解为 3-酮-DON 之前生成 DON。这些结果为利用岛状德沃斯氏菌 A16 作为生物降解剂来控制谷物中的 DON 污染提供了潜力。
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