Animal and Bioscience Research Department, Teagasc Grange, Dunsany, C15 PW93, Co. Meath, Ireland.
Laboratory of Animal Reproduction, Department of Biological Sciences, University of Limerick, V94 T9PX, Limerick, Ireland.
Sci Rep. 2018 Nov 8;8(1):16577. doi: 10.1038/s41598-018-34611-4.
The aim of this study was to investigate the effect of early calf-hood nutrition on the transcriptomic profile of the arcuate nucleus of the hypothalamus, anterior pituitary and testes in Holstein-Friesian bulls. Holstein-Friesian bull calves with a mean (±S.D.) age and bodyweight of 19 (±8.2) days and 47.5 (±5.3) kg, respectively, were offered a high (n = 10) or low (n = 10) plane of nutrition in order to achieve an overall growth rate of 1.2 and 0.5 kg/day. At 126 (±3) days of age, calves were euthanized, hypothalamus (arcuate region), anterior pituitary and testicular parenchyma samples were harvested and RNAseq analysis was performed. There were 0, 49 and 1,346 genes differentially expressed in the arcuate nucleus, anterior pituitary and testicular tissue of bull calves on the low relative to the high plane of nutrition, respectively (P < 0.05; False Discovery Rate <0.05). Cell cycle processes in the anterior pituitary were down regulated in the low relative to the high plane of nutrition; there was no differential expression of genes related to reproductive processes. Gene expression involved in cholesterol and androgen biosynthesis in the testes were down regulated in animals on the low plane of nutrition. This study provides insight into the effect of early life plane of nutrition on the regulation of the HPT axis.
本研究旨在探讨早期牛犊饲养对荷斯坦-弗里森公牛弓状核、垂体前叶和睾丸转录组谱的影响。选择平均（±标准差）年龄和体重分别为 19（±8.2）天和 47.5（±5.3）kg 的荷斯坦-弗里森公牛犊牛，分别提供高（n=10）或低（n=10）营养水平，以实现 1.2 和 0.5kg/d 的总生长速度。在 126（±3）天龄时，对犊牛进行安乐死，采集下丘脑（弓状区）、垂体前叶和睾丸实质样本，并进行 RNAseq 分析。在低营养水平下，牛犊的弓状核、垂体前叶和睾丸组织中分别有 0、49 和 1346 个基因表达存在差异（P<0.05；错误发现率<0.05）。垂体前叶的细胞周期过程在低营养水平下被下调；与生殖过程相关的基因无差异表达。睾丸中胆固醇和雄激素生物合成相关基因的表达在低营养水平下下调。本研究深入了解了早期生活营养水平对 HPT 轴调节的影响。
