Schalow G, Schmidt H
Pflugers Arch. 1977 Nov 25;372(1):17-22. doi: 10.1007/BF00582201.
A. Pyriformis and extensor longus digiti IV muscles of Rana temporaria were denervated by cutting the sciatic or peroneal nerve at various distances from the muscles. Slow fibres were identified by their membrane time constants, and examined for their ability to produce action potentials in response to intracellularly applied current pulses. B. The slow muscle fibres acquired the ability to generate action potentials several days after denervation. The duration of this latent period depended on the length of the peripheral nerve stump, and on the temperature at which the frogs were kept after the operation. C. At 18 degrees C the latent period increased by 0.36 days per mm of sciatic nerve stump. At 11.5 degrees C the corresponding value was 0.7 days/mm. The effect of length of the peroneal nerve was smaller than that of the sciatic nerve. D. It is suggested that the peripheral nerve stump serves as a reservoir of 'trophic' material which is transported towards the slow fibres at a rate of 2.8 mm/day (at 18 degrees C) and seems to block the formation of Na channels. The Q10 value of this transport system would be 2.7.
A. 通过在距肌肉不同距离处切断坐骨神经或腓总神经，使林蛙的梨状肌和第四趾长伸肌失神经支配。通过膜时间常数识别慢肌纤维，并检测其对细胞内施加电流脉冲产生动作电位的能力。B. 失神经支配几天后，慢肌纤维获得了产生动作电位的能力。这个潜伏期的持续时间取决于外周神经残端的长度以及手术后青蛙所处的温度。C. 在18℃时，潜伏期每增加1毫米坐骨神经残端，增加0.36天。在11.5℃时，相应的值为0.7天/毫米。腓总神经长度的影响小于坐骨神经。D. 有人提出，外周神经残端充当“营养”物质的储存库，这些物质以2.8毫米/天（在18℃时）的速度向慢肌纤维运输，并且似乎会阻止钠通道的形成。这个运输系统的Q10值为2.7。
