系统性红斑狼疮患者中杀伤细胞免疫球蛋白样受体 3DL1 的自身抗体。
Autoantibodies to killer cell immunoglobulin-like receptor 3DL1 in patients with systemic lupus erythematosus.
机构信息
Department of Intractable Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
出版信息
Clin Exp Immunol. 2019 Mar;195(3):358-363. doi: 10.1111/cei.13235. Epub 2018 Dec 14.
A genetic variant of the killer immunoglobulin-like receptor 3DL1 (KIR3DL1) has been found in patients with systemic lupus erythematosus (SLE). Herein, we investigated the presence of autoantibodies to KIR3DL1 in a cohort of patients with SLE. We tested sera from 28 patients with SLE, 11 patients with rheumatoid arthritis (RA) and 17 healthy control subjects for anti-KIR3DL1 activity by an enzyme-linked immunosorbent assay (ELISA) using recombinant KIR3DL1-enhanced green fluorescent protein (EGFP) and EGFP proteins. Anti-KIR3DL1 antibodies were detected in 22 (79%) of the 28 patients with SLE, whereas they were present in only three (27%) of the 11 patients with RA examined. Notably, 10 (91%) of the 11 samples from patients with SLE prior to therapy had anti-KIR3DL1 antibodies. None of the samples from healthy donors were positive for the antibodies. Here, we report the presence of anti-KIR3DL1 antibodies in the sera of patients with SLE for the first time. Anti-KIR3DL1 autoantibodies may be involved in the pathogenesis of autoimmune diseases.
一种杀伤免疫球蛋白样受体 3DL1（KIR3DL1）的遗传变异已在系统性红斑狼疮（SLE）患者中被发现。在此，我们研究了 SLE 患者群体中存在针对 KIR3DL1 的自身抗体。我们通过酶联免疫吸附试验（ELISA）使用重组 KIR3DL1 增强型绿色荧光蛋白（EGFP）和 EGFP 蛋白检测了来自 28 名 SLE 患者、11 名类风湿关节炎（RA）患者和 17 名健康对照者的血清中的抗 KIR3DL1 活性。我们在 28 名 SLE 患者中的 22 名（79%）中检测到抗 KIR3DL1 抗体，而在 11 名 RA 患者中的 3 名（27%）中存在抗 KIR3DL1 抗体。值得注意的是，在治疗前的 11 份 SLE 患者样本中，有 10 份（91%）存在抗 KIR3DL1 抗体。健康供体的样本均未检测到抗体。在这里，我们首次报道了 SLE 患者血清中存在抗 KIR3DL1 抗体。抗 KIR3DL1 自身抗体可能参与了自身免疫性疾病的发病机制。
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