基于阳离子柱[5]芳烃修饰的金纳米粒子的新型竞争性荧光传感平台用于检测左旋肉碱。
Novel Competitive Fluorescence Sensing Platform for L-carnitine Based on Cationic Pillar[5]Arene Modified Gold Nanoparticles.
机构信息
Key Lab of Inorganic Special Functional Materials, Chongqing Municipal Education Commission, School of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling 408100, China.
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650224, China.
出版信息
Sensors (Basel). 2018 Nov 14;18(11):3927. doi: 10.3390/s18113927.
Supramolecular host-guest interaction and sensing between cationic pillar[5]arenes (CP5) and L-carnitine were developed by the competitive host-guest recognition for the first time. The fluorescence sensing platform was constructed by CP5 functionalized Au nanoparticles (CP5@Au-NPs) as receptor and probe (rhodamine 123, R123), which shown high sensitivity and selectivity for L-carnitine detection. Due to the negative charge and molecular size properties of L-carnitine, it can be highly captured by the CP5 via electrostatic interactions and hydrophobic interactions. The host-guest mechanism between PP5 and L-carnitine was studied by ¹H NMR and molecular docking, indicating that more affinity binding force of CP5 with L-carnitine. Therefore, a selective and sensitive fluorescent method was developed. It has a linear response of 0.1⁻2.0 and 2.0⁻25.0 μM and a detection limit of 0.067 μM (S/N = 3). The fluorescent sensing platform was also used to detect L-carnitine in human serum and milk samples, which provided potential applications for the detection of drugs abuse and had path for guarding a serious food safety issues.
首次通过竞争性主客体识别开发了阳离子柱[5]芳烃（CP5）与左旋肉碱之间的超分子主体-客体相互作用和传感。荧光传感平台由 CP5 功能化的金纳米粒子（CP5@Au-NPs）作为受体和探针（罗丹明 123，R123）构建，对左旋肉碱的检测表现出高灵敏度和选择性。由于左旋肉碱带负电荷和分子尺寸的特性，它可以通过静电相互作用和疏水相互作用被 CP5 高度捕获。通过¹H NMR 和分子对接研究了 CP5 与左旋肉碱之间的主客体机制，表明 CP5 与左旋肉碱具有更强的结合力。因此，开发了一种选择性和灵敏的荧光方法。它在 0.1⁻2.0 和 2.0⁻25.0 μM 范围内具有线性响应，检测限为 0.067 μM（S/N = 3）。荧光传感平台还用于检测人血清和牛奶样品中的左旋肉碱，为药物滥用检测提供了潜在的应用，并为严重的食品安全问题提供了保障途径。
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