Tash Rehab M Elsaid, Wegdan Ahmed A, Amer Fatma A, Bassyouni Rasha H A, Botros Joseph M
Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Indian J Anaesth. 2018 Oct;62(10):786-792. doi: 10.4103/ija.IJA_41_18.
Infection control is essential in anaesthetic practice for both personnel and equipment used. This study aims to evaluate knowledge of anaesthesiologists about infection control practices and to detect the pattern of anaesthetic devices contamination.
Cross-sectional observational study at two university hospitals was done. Self-administered questionnaires were distributed to 80 anaesthesiologists and 90 nursing staff. Forty-four samples were taken from rigid laryngoscopes (22 pairs from handle and blade) for detection of bacterial or fungal contamination. Same laryngoscopes were tested for occult blood.
The response rate among the physicians was 72% while for nurses 94.4%. The responses were variable reflecting lack of adequate knowledge and unsatisfactory compliance to infection control practices. Tested samples showed no fungal growth. Fourteen (31.8%) samples were negative for bacteriological contamination and 5/44 (11.4%) showed gram-positive bacilli; gram-positive cocci were isolated from 12 samples (27.3%) where and , respectively, shared 18.2% and 9.1% of the total samples. Gram-negative bacilli were isolated from 13 samples (29.5%), of which spp. were most frequent (11.4%). Both and were isolated from 6.8% each. spp. was isolated from 4.5%. Occult blood was found in 45.5% of samples.
The current study showed contamination of ready-to-use laryngoscopes in operative theatres and ICUs.
在麻醉实践中，感染控制对于所使用的人员和设备至关重要。本研究旨在评估麻醉医生对感染控制措施的了解情况，并检测麻醉设备的污染模式。
在两家大学医院开展了横断面观察性研究。向80名麻醉医生和90名护理人员发放了自填式问卷。从硬式喉镜（22对，包括手柄和镜片）采集了44个样本，用于检测细菌或真菌污染。对同一喉镜进行潜血检测。
医生的回复率为72%，护士为94.4%。回复情况各不相同，反映出知识不足以及对感染控制措施的依从性不尽人意。检测样本未显示真菌生长。14个（31.8%）样本细菌学污染呈阴性，5/44（11.4%）显示革兰氏阳性杆菌；从12个样本（27.3%）中分离出革兰氏阳性球菌，其中 和 分别占总样本的18.2%和9.1%。从13个样本（29.5%）中分离出革兰氏阴性杆菌，其中 属最为常见（11.4%）。 和 各占6.8%。 属占4.5%。45.5%的样本中发现潜血。
当前研究显示，手术室和重症监护病房中即用型喉镜存在污染。
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