Discipline for Biosciences and Bio-Medical Engineering, Indian Institute of Technology Indore, Simrol Indore 453552, India.
Chem Commun (Camb). 2019 Jan 2;55(3):294-297. doi: 10.1039/c8cc07429a.
The morphological divergence of the Endoplasmic Reticulum (ER) during stress is a powerful indicator of several diseases. A new two-photon, non-cytotoxic, fluorescent probe (ERLp) was designed and synthesized in a facile manner for selective tracking of the Endoplasmic Reticulum (ER) with a high Pearson co-localization coefficient (0.91) in live cells and tumor spheroids. Further, ER stress during cell apoptosis and vesicular transport from the ER to the lysosomal compartment were also explored by employing ERLp. Therefore, ERLp can be used as a potent tool for examining vesicle transport or ER stress associated diseases in real time.
内质网（ER）在应激过程中的形态变化是几种疾病的有力指标。本研究设计并合成了一种新型双光子、非细胞毒性、荧光探针（ERLp），该探针能够以高皮尔逊共定位系数（0.91）在活细胞和肿瘤球体中选择性追踪内质网。此外，还利用 ERLp 研究了细胞凋亡过程中的内质网应激以及从内质网到溶酶体腔的囊泡运输。因此，ERLp 可以作为一种有效的工具，用于实时研究与囊泡运输或内质网应激相关的疾病。
