Department of Basic Neurosciences, University of Geneva, 1211 Geneva, Switzerland
Clinic of Neurology, Geneva University Hospital, Geneva, Switzerland.
Development. 2018 Nov 19;145(22):dev172049. doi: 10.1242/dev.172049.
The field of developmental neuroscience is benefitting from recent technological advances that allow access to organogenesis via organoid preparations. These methods have been applied to better understanding neural identity, and have opened up a window into the early events that occur during development of the human brain. However, current approaches are not without their limitations, and although brain organoids and other paradigms recapitulate many processes with remarkable fidelity, there are clear differences between brain organoid development and brain development These topics were discussed extensively at a recent workshop organized by The Company of Biologists entitled 'Thinking beyond the dish: taking neural differentiation to the next level'. Here, we summarize the common themes that emerged from the workshop and highlight some of the limitations and the potential of this emerging technology. In particular, we discuss how organoids can help us understand not only healthy and diseased brain, but also explore new arrays of cellular behaviors.
发育神经科学领域受益于最近的技术进步，这些进步使人们可以通过类器官制备来研究器官发生。这些方法已被应用于更好地理解神经特征，并为了解人类大脑发育过程中早期发生的事件提供了一个窗口。然而，目前的方法并非没有局限性，尽管脑类器官和其他模型能够以惊人的准确性再现许多过程，但脑类器官发育和大脑发育之间存在明显的差异。这些主题在最近由 The Company of Biologists 组织的题为“超越培养皿思考：将神经分化提升到一个新的水平”的研讨会上进行了广泛的讨论。在这里，我们总结了研讨会中出现的共同主题，并强调了这项新兴技术的一些局限性和潜力。特别是，我们讨论了类器官如何帮助我们不仅了解健康和患病的大脑，还探索新的细胞行为阵列。
