通过SNaPshot™基因分型确定木曾马毛色相关的MC1R、ASIP和MATP基因的变异情况。
Variation in the MC1R, ASIP, and MATP genes responsible for coat color in Kiso horse as determined by SNaPshot™ genotyping.
作者信息
Nakamura Kotono, Tozaki Teruaki, Kakoi Hironaga, Owada Satsuki, Takasu Masaki
机构信息
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
出版信息
J Vet Med Sci. 2019 Jan 8;81(1):100-102. doi: 10.1292/jvms.18-0458. Epub 2018 Nov 22.
Kiso horse is a breed of Japanese native horses. In this study, to clarify coat color gene variation in Kiso horses, we used SNaPshot™ genotyping to evaluate variation in MC1R, ASIP, and MATP genes at the Extension (E), Agouti (A), and Cream dilution (C) loci. The coat color of 149 horses was documented. The coat color of 140, 3, and 6 horses was bay, chestnut, and buckskin, respectively. Furthermore, the frequency of alleles E, e, A, a, C, and Cr was 0.80, 0.20, 0.86, 0.14, 0.98, and 0.02, respectively. Current status of coat color genes in Kiso horses was clarified, and this information will help plan further conservation of the horses.
木曾马是日本本土马的一个品种。在本研究中，为了阐明木曾马的毛色基因变异，我们使用SNaPshot™基因分型技术评估了扩展（E）、刺豚鼠信号蛋白（A）和乳白稀释（C）位点的黑素皮质素受体1（MC1R）、刺豚鼠信号蛋白（ASIP）和膜相关转运蛋白（MATP）基因的变异。记录了149匹马的毛色。其中140匹、3匹和6匹马的毛色分别为枣色、栗色和黄棕色。此外，等位基因E、e、A、a、C和Cr的频率分别为0.80、0.20、0.86、0.14、0.98和0.02。阐明了木曾马毛色基因的现状，这些信息将有助于规划该马种的进一步保护工作。
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