Department of Physiology, Serampore College, Serampore, India.
Arch Physiol Biochem. 2020 Oct;126(4):348-355. doi: 10.1080/13813455.2018.1538249. Epub 2018 Nov 23.
Alteration of redox signalling and RANK-L expression in FBMCs of mice exposed to different intensities of cold stress (15 °C, 8 °C and 4 °C) were studied. To understand the effects of varying intensities of cold stress on murine FBMCs and its impact on osteoclastogenesis. FBMCs were isolated from mice exposed to different intensities of cold stress and used for immunoblotting and biochemical assays. Bone histometry was also done. Different intensities of cold stress perturb redox signalling in FBMCs and alters bone histometry. Higher RANK-L expressions were noted in FBMCs of mice exposed to 8 °C and 4 °C as compared with 15 °C. Cold stress boosts free radical production in FBMC's, which might enhance RANK-L expression, an indicator of osteoclastogenesis. Thus, we speculate that stronger cold stress (8 °C and 4 °C) contributes to the development of early bone loss.
研究了不同强度冷应激（15°C、8°C 和 4°C）暴露下的小鼠 FBMC 中氧化还原信号和 RANK-L 表达的变化。为了了解不同强度冷应激对小鼠 FBMC 的影响及其对破骨细胞生成的影响。从暴露于不同强度冷应激的小鼠中分离 FBMC 用于免疫印迹和生化分析。还进行了骨计量学分析。不同强度的冷应激会扰乱 FBMC 中的氧化还原信号，并改变骨计量学。与 15°C 相比，8°C 和 4°C 暴露的小鼠 FBMC 中 RANK-L 的表达更高。冷应激会增加 FBMC 中自由基的产生，这可能会增强 RANK-L 的表达，RANK-L 是破骨细胞生成的指标。因此，我们推测更强的冷应激（8°C 和 4°C）会导致早期骨丢失的发展。
