Nie Qian, Yang Lan, Qing Wenjie, Xie Jie, Gong Xiaodong, Chen Huimin, Gan Yuwen, Wang Ling, Xiang Jia-Wen, Xiao Yuan, Chen Zhigang, Li David Wan-Cheng
Key Laboratory of Protein Chemistry and Developmental Biology, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
The State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, #54 Xianlie South Road, Guangzhou, Guangdong 510060, China.
Curr Mol Med. 2018;18(6):376-382. doi: 10.2174/1566524018666181116112208.
It is now well established that protein sumoylation is an important mechanism to regulate multiple cellular processes including gene transcription, chromatin structure, cell proliferation and differentiation, as well as pathogenesis.
In the vertebrate eye, we and others have previously shown that sumoylation can regulate differentiation of major ocular tissues including retina and lens. However, the expression patterns of the three types of sumoylation enzymes, the activating enzymes SAE1 and UBA2, the conjugating enzyme UBC9, and the ligating enzymes such as RanBP2 and PIAS1 have not been well studied in the ocular tissues.
In the present study, using QRT-PCR and western blot analysis, we have determined the differentiatial expression patterns of the above three types of enzymes, and the obtained results lay down a foundation for further exploration of sumoylation functions in vertebrate eye.
目前已充分证实，蛋白质的类泛素化修饰是一种重要机制，可调节包括基因转录、染色质结构、细胞增殖与分化以及发病机制在内的多种细胞过程。
在脊椎动物眼中，我们和其他研究人员先前已表明，类泛素化修饰可调节包括视网膜和晶状体在内的主要眼组织的分化。然而，三种类型的类泛素化修饰酶，即激活酶SAE1和UBA2、缀合酶UBC9以及连接酶如RanBP2和PIAS1在眼组织中的表达模式尚未得到充分研究。
在本研究中，我们通过实时定量聚合酶链反应（QRT-PCR）和蛋白质免疫印迹分析，确定了上述三种类型酶的差异表达模式，所得结果为进一步探索类泛素化修饰在脊椎动物眼中的功能奠定了基础。
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