Teixeira Lucas Novaes, Janner Évelin Caroline, Teixeira Thaís, Passador-Santos Fabrício, Martinez Elizabeth Ferreira, Demasi Ana Paula Dias, de Araújo Ney Soares, de Araújo Vera Cavalcanti
1 São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil.
Int J Surg Pathol. 2019 Jun;27(4):360-371. doi: 10.1177/1066896918813678. Epub 2018 Nov 27.
The present study aimed to compare the expression of p63/p40 with smooth muscle actin (SMA) and vimentin (VIM) by myoepithelial cells in minor salivary gland tumors. Fifty-two formalin-fixed paraffin-embedded samples of minor salivary gland tumors derived from intercalated duct (pleomorphic adenoma [PA], adenoid cystic carcinoma [ACC], epithelial-myoepithelial carcinoma [EMC], polymorphous adenocarcinoma [PAC], and secretory carcinoma [SC]) and 3 samples of minor salivary gland tumors derived from excretory duct (mucoepidermoid carcinoma [MEC]) were evaluated by means of immunohistochemistry. The data were analyzed qualitatively. The results indicated that p63 and p40 expression were detected in myoepithelial cells present in PA, ACC, and EMC. However, both proteins were also observed in squamous areas of PA and all cases of MEC. SMA were noticed in some myoepithelial cells of PA, ACC, and EMC. Expression of SMA was negative in the other salivary gland tumors evaluated. VIM was constantly expressed by myoepithelial cells in PA, ACC, and EMC. VIM was also observed in cells of PAC and SC, but not in squamous areas of PA and MEC. In conclusion, p63 expression is almost comparable with VIM in detecting myoepithelial cells, an immunolabeling pattern not followed by p40, and consequently, caution has to be taken during the interpretation of salivary gland tumor exhibiting an p63/p40 phenotype in order to avoid a misdiagnosis.
本研究旨在比较小涎腺肿瘤中肌上皮细胞的p63/p40与平滑肌肌动蛋白（SMA）和波形蛋白（VIM）的表达情况。采用免疫组织化学方法对52例源自闰管的小涎腺肿瘤（多形性腺瘤[PA]、腺样囊性癌[ACC]、上皮-肌上皮癌[EMC]、多形性腺癌[PAC]和分泌性癌[SC]）的福尔马林固定石蜡包埋样本以及3例源自排泄管的小涎腺肿瘤（黏液表皮样癌[MEC]）样本进行评估。对数据进行定性分析。结果表明，在PA、ACC和EMC的肌上皮细胞中检测到p63和p40表达。然而，在PA的鳞状区域和所有MEC病例中也观察到这两种蛋白。在PA、ACC和EMC的一些肌上皮细胞中发现有SMA。在所评估的其他涎腺肿瘤中，SMA表达为阴性。VIM在PA、ACC和EMC的肌上皮细胞中持续表达。在PAC和SC的细胞中也观察到VIM，但在PA和MEC的鳞状区域未观察到。总之，在检测肌上皮细胞方面，p63表达与VIM几乎相当，而p40则不具有这种免疫标记模式，因此，在解读表现出p63/p40表型的涎腺肿瘤时必须谨慎，以避免误诊。
