帕西瑞肽对大鼠模型中接受和未接受全身照射情况下肠吻合口愈合的影响。

The effect of pasireotide on intestinal anastomotic healing with and without whole-body irradiation in a rat model.

作者信息

Seifert Gabriel J, Leithold Gunnar, Kulemann Birte, Holzner Philipp A, Glatz Torben, Hoeppner Jens, Kirste Simon, Marjanovic Goran, Laessle Claudia

机构信息

Department of General and Visceral Surgery, Medical Centre - University of Freiburg, Faculty of Medicine, University Freiburg, Hugstetter Str. 55, 79106, Freiburg, Germany.

Department of Radiooncology, Medical Centre - University of Freiburg, Faculty of Medicine, University Freiburg, Robert-Koch-Str. 3, 79106, Freiburg, Germany.

出版信息

Int J Colorectal Dis. 2019 Feb;34(2):337-345. doi: 10.1007/s00384-018-3193-5. Epub 2018 Nov 27.

DOI:10.1007/s00384-018-3193-5

PMID:30483864

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6331742/

Abstract

OBJECTIVE

To examine pasireotide's effect on intestinal anastomotic healing under physiological conditions and following preoperative whole-body irradiation.

MATERIAL AND METHODS

Forty-five male Wistar rats received an ileoileal end-to-end anastomosis. Group 1 (Co, n = 9) served as control. Group 2 (SOM, n = 10) received pasireotide (60 mg/kg) 6 days preoperatively. Group 3 (R-Co, n = 13) was subjected to 8 Gy whole-body irradiation 4 days preoperatively. Finally, group 4 (R-SOM, n = 13) received pasireotide 6 days preoperatively and whole-body irradiation 4 days preoperatively. On postoperative day 4, anastomotic bursting pressure, histology, IGF-1 staining, and collagen density were examined.

RESULTS

Mortality was higher in irradiated animals (30.8% vs. 5.3%, p = 0.021), and anastomotic bursting pressure was significantly lower (median, R-Co = 83 mmHg; R-SOM = 101 mmHg; Co = 149.5 mmHg; SOM = 169 mmHg). Inflammation measured by leukocyte infiltration following irradiation was reduced (p = 0.023), and less collagen was observed, though this was not statistically significant. Bursting pressure did not significantly differ between Co and SOM and between R-Co and R-SOM animals respectively. Semi-quantitative scoring of IGF-1, fibroblast bridging, or collagen density did not reveal significant differences among the groups.

CONCLUSION

Whole-body irradiation decreases the quality of intestinal anastomotic wound healing and increases mortality. Pasireotide does not significantly lessen this detrimental effect.

摘要

目的

研究帕西瑞肽在生理条件下以及术前全身照射后对肠道吻合口愈合的影响。

材料与方法

45只雄性Wistar大鼠接受回肠端端吻合术。第1组（对照组，n = 9）作为对照。第2组（SOM组，n = 10）在术前6天接受帕西瑞肽（60 mg/kg）。第3组（R-Co组，n = 13）在术前4天接受8 Gy全身照射。最后，第4组（R-SOM组，n = 13）在术前6天接受帕西瑞肽，在术前4天接受全身照射。术后第4天，检测吻合口破裂压力、组织学、IGF-1染色和胶原密度。

结果

照射动物的死亡率更高（30.8% 对5.3%，p = 0.021），吻合口破裂压力显著更低（中位数，R-Co组 = 83 mmHg；R-SOM组 = 101 mmHg；对照组 = 149.5 mmHg；SOM组 = 169 mmHg）。照射后通过白细胞浸润测量的炎症减轻（p = 0.023），观察到的胶原较少，尽管这无统计学意义。对照组与SOM组之间以及R-Co组与R-SOM组动物之间的破裂压力无显著差异。IGF-1、成纤维细胞桥接或胶原密度的半定量评分在各组之间未显示出显著差异。