与猪背最长肌肌肉内肉质变异相关的蛋白质组差异丰度。
Differential abundance of proteome associated with intramuscular variation of meat quality in porcine longissimus thoracis et lumborum muscle.
机构信息
Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of Korea; Institutes of Green-Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea.
Institutes of Green-Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea.
出版信息
Meat Sci. 2019 Mar;149:85-95. doi: 10.1016/j.meatsci.2018.11.012. Epub 2018 Nov 15.
Intramuscular variation of meat quality in the porcine longissimus thoracis et lumborum (LTL) muscle was evaluated by assessing the differential abundance of proteome components. Twenty LTL muscles were individually divided into three regions (anterior, medial, and posterior) according to meat color. CIE L and b were higher (P < 0.05), but Warner-Bratzler shear force (WBSF) was lower (P < 0.05) in the anterior region, where myosin-1 and -2, TPM2, MLC1f, MLC2, Hsp27, and TPI1 were highly (P < 0.05) abundant. However, CIE a, drip loss, and WBSF were higher (P < 0.05) in the medial region. Glycolysis enzymes including enolase 3, ALDOA, LDHA, PGM1, and TPI1 were highly abundant in the medial and posterior region, whereas GAPDH and myoglobin were overexpressed in the medial region (P < 0.05). Therefore, intramuscular variations in color, water-holding capacity, and tenderness were associated with differential abundance of the proteome, especially contractile, glycolysis enzymes, and Hsp27.
采用蛋白质组差异丰度评估方法研究了猪背最长肌（LTL）肌肉内的肉质变化。根据肉色，将 20 块 LTL 肌肉分别分为三个区域（前、中、后）。前区的 CIE L 和 b 值较高（P < 0.05），但 Warner-Bratzler 剪切力（WBSF）较低（P < 0.05），其中肌球蛋白-1 和 -2、TPM2、MLC1f、MLC2、Hsp27 和 TPI1 高度丰富（P < 0.05）。然而，中区的 CIE a、滴水损失和 WBSF 较高（P < 0.05）。中、后区糖酵解酶（烯醇酶 3、ALDOA、LDHA、PGM1 和 TPI1）高度丰富，而中区的 GAPDH 和肌红蛋白表达上调（P < 0.05）。因此，颜色、保水性和嫩度的肌内变化与蛋白质组的差异丰度有关，特别是收缩、糖酵解酶和 Hsp27。
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