Esmaeili M, Golhasan B, Ye W, Heydari R
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture and Consumer Services, Raleigh, NC 27607, USA.
J Helminthol. 2018 Nov 29;94:e16. doi: 10.1017/S0022149X18001037.
A new species of Paurodontoides, P. siddiqii n. sp., is described and illustrated based on its morphological, morphometric, and molecular characters. The new species is characterized by a female 550-729 μm long, lip region continuous with body contour, stylet length 7.0-8.0 μm long or c. 1.0-1.2 times the lip region diameter, lateral fields with four smooth incisures, excretory pore at 85-125 μm from anterior end located at the base of the pharyngeal bulb or posterior to it, basal pharyngeal bulb with a short posterior extension projecting into the intestine, monodelphic-prodelphic reproductive system with prominent 19-22 μm long post-uterine sac, and elongate conoid tail with a filiform terminus. The new species is compared with two known species of the genus. It differs from the type species of the genus, P. linfordi, by having slightly shorter stylet, lateral field with smooth incisures, different position of the excretory pore, and absence of male. Compared to P. latus, the new species has a shorter body, shorter stylet, different position of the excretory pore, female tail shape and absence of male. The new species was also compared with close species of the genus Paurodontus because of lateral field marked with four lines, asymmetrical stylet knobs and absence of male. Molecular phylogenetic studies of the new species using partial sequences of 18S rDNA revealed that it forms a clade with a species of the genus Ficotylus. In phylogenetic analyses using partial sequences of the 28S rDNA D2-D3 domain, the new species formed a monophyletic group with a species of the genus Veleshkinema and Sphaerularia spp. (Sphaerulariinae).
基于形态学、形态测量学和分子特征，描述并图示了一种新的类拟短体线虫属物种——西迪基拟短体线虫（Paurodontoides siddiqii n. sp.）。新物种的特征为：雌虫体长550 - 729μm，唇区与虫体轮廓连续，口针长7.0 - 8.0μm，约为唇区直径的1.0 - 1.2倍，侧区有四条平滑的刻线，排泄孔位于距前端85 - 125μm处，在咽球基部或其后方，咽球基部有一短的向后延伸部分伸入肠内，单雌型 - 前雌型生殖系统，有明显的19 - 22μm长的子宫后囊，尾部长而呈锥形，末端丝状。将新物种与该属的两个已知物种进行了比较。它与该属的模式种林福德拟短体线虫（P. linfordi）不同，口针稍短，侧区有平滑刻线，排泄孔位置不同，且无雄虫。与宽拟短体线虫（P. latus）相比，新物种身体较短，口针较短，排泄孔位置不同，雌虫尾部形状不同，且无雄虫。由于侧区有四条线、口针瘤不对称且无雄虫，新物种还与短体线虫属的近缘物种进行了比较。使用18S rDNA部分序列对新物种进行的分子系统发育研究表明，它与菲科蒂线虫属（Ficotylus）的一个物种形成一个分支。在使用28S rDNA D2 - D3区域部分序列进行的系统发育分析中，新物种与韦列什金线虫属（Veleshkinema）的一个物种和球孢线虫属（Sphaerularia spp.）（球孢线虫亚科）形成一个单系群。
