Concanavalin A-sugar affinity based system: Binding interactions, principle of glucose-responsiveness, and modulated insulin release for diabetes care.

The glucose-responsive behavior of the Con A-sugar affinity based hydrogels, microgels, films and nanoparticles has been widely investigated in the application of drug delivery and biosensors. However, the principle or mechanism of such systems is not well known in literature. In this study, for the first time, the glucose-responsive principle (or principle) of the Con A-sugar based system was detailedly explained through the binding interactions test by isothermal titration calorimetry and ligand binding theory. The study also successfully resolved the controversy regarding the principle of such systems in literature. The other contribution of the study is the experimental validation that the insulin-loaded microgels with different hydrogel-network compositions (based on Con A-sugar affinity) respond to different glucose concentrations. Particularly, the result of the in vitro insulin release suggests that the microgels be able to maintain bolus and basal insulin release in response to different glucose concentrations and the network composition be able to affect the burst release, release rate and overall release amount of insulin. Further, the released insulin has been shown to remain active and the microgels possess no in vitro cytotoxicity to HDF cells. The second contribution is a first step towards the realization of personalized diabetes care.