贵州育龄人群中HbA和HbF的参考区间及β地中海贫血携带者筛查的HbA临界值

Reference intervals for HbA and HbF and cut-off value of HbA for β-thalassemia carrier screening in a Guizhou population of reproductive age.

作者信息

Han Wen-Ping, Huang Ling, Li Yuan-Yuan, Han Yuan-Yuan, Li Di, An Bang-Quan, Huang Sheng-Wen

机构信息

Department of Laboratory, Guizhou Provincial People's Hospital, No.83 East Zhongshan Road, Guiyang 550002, Guizhou, PR China; Nanjing Red Cross Blood Center, Nanjing 210003, Jiangsu, PR China.

Department of Laboratory, Guizhou Provincial People's Hospital, No.83 East Zhongshan Road, Guiyang 550002, Guizhou, PR China.

出版信息

Clin Biochem. 2019 Mar;65:24-28. doi: 10.1016/j.clinbiochem.2018.11.007. Epub 2018 Nov 29.

DOI:10.1016/j.clinbiochem.2018.11.007

Abstract

OBJECTIVE

The aims of this study were to establish the reference intervals for HbA2 and HbF in a Guizhou population of reproductive age, and to determine the cut-off value of HbA for β-thalassemia carrier screening.

METHODS

Hemoglobin analysis was performed on 832 individuals without hypochromic microcytic anemia to calculate the reference intervals for HbA and HbF. Three hundred and ninety one β-thalassemia carriers and non β-thalassemia individuals were analyzed for their HbA levels followed by detecting β-globin gene mutations, then cut-off value of HbA for β-thalassemia carrier screening was determined using ROC curve analysis.

RESULTS

The reference interval for HbA in overall normal individuals was 2.3%-3.1%, and reference intervals for HbF in normal males and females (including normal females and pregnant women) were 0-0.5% and 0-1.0% respectively. The cut-off values of HbA for β-thalassemia carrier screening in males, non-pregnant women, pregnant women and the overall set were 4.40%, 3.75%, 3.70% and 3.95% respectively.

CONCLUSION

Gender and pregnancy status had no obvious influence on reference interval for HbA. The HbF level was higher in females than in males, but pregnancy status had no obvious influence on HbF level. Cut-off value of HbA for β-thalassemia carrier screening was obviously affected by gender but not by pregnancy status.

摘要

目的

本研究旨在建立贵州育龄人群中HbA2和HbF的参考区间，并确定用于β地中海贫血携带者筛查的HbA截断值。

方法

对832例无低色素小细胞性贫血的个体进行血红蛋白分析，以计算HbA和HbF的参考区间。分析391例β地中海贫血携带者和非β地中海贫血个体的HbA水平，随后检测β珠蛋白基因突变，然后使用ROC曲线分析确定用于β地中海贫血携带者筛查的HbA截断值。

结果

总体正常个体HbA的参考区间为2.3%-3.1%，正常男性和女性（包括正常女性和孕妇）HbF的参考区间分别为0-0.5%和0-1.0%。男性、非孕妇、孕妇及总体人群中用于β地中海贫血携带者筛查的HbA截断值分别为4.40%、3.75%、3.70%和3.95%。

结论

性别和妊娠状态对HbA参考区间无明显影响。女性HbF水平高于男性，但妊娠状态对HbF水平无明显影响。用于β地中海贫血携带者筛查的HbA截断值受性别影响明显，但不受妊娠状态影响。