Barczewska Monika, Jezierska-Wozniak Katarzyna, Habich Aleksandra, Lipinski Seweryn, Holak Piotr, Maksymowicz Wojciech, Wojtkiewicz Joanna
Folia Neuropathol. 2018;56(2):124-132. doi: 10.5114/fn.2018.76616.
The pathophysiology of degenerative disc disease (DDD) is complex and not fully understood. While surgical treatment and appropriate rehabilitation offer relief of acute symptoms, there is a need to find tissue engineering strategies for intervertebral disc repair to restore healthy higher and histological structure. The purpose of this study was to estimate the survival rate of transplanted cells and their post-delivery integration level at the damage site.
We used an porcine model to investigate autogenic bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation for intervertebral disc repair. In our experiment we used a large animal model of DDD induced by percutaneous laser light deliveries. The percutaneous approach has also been used for delivery of BM-MSCs into the intervertebral disc space.
After MSC transplantation, we observed a deceleration of the degenerative process in the intervertebral disc, relative to degenerative discs without MSC transplantation.
By using a large animal model that mimicked the development of intervertebral degenerative disc disease, the present results are indicative of the clinical feasibility of this procedure.
退行性椎间盘疾病（DDD）的病理生理学很复杂，尚未完全了解。虽然手术治疗和适当的康复可以缓解急性症状，但仍需要找到用于椎间盘修复的组织工程策略，以恢复健康的更高层次结构和组织学结构。本研究的目的是评估移植细胞的存活率及其在损伤部位的递送后整合水平。
我们使用猪模型研究自体骨髓间充质干细胞（BM-MSC）移植用于椎间盘修复。在我们的实验中，我们使用了经皮激光照射诱导的DDD大型动物模型。经皮方法也已用于将BM-MSCs递送到椎间盘间隙。
与未进行MSC移植的退变椎间盘相比，MSC移植后，我们观察到椎间盘退变过程减缓。
通过使用模拟椎间盘退变疾病发展的大型动物模型，目前的结果表明该手术具有临床可行性。
