用于确定BCL-2家族蛋白质结构与相互作用的连续波电子顺磁共振和双电子-电子共振方法：定点自旋标记在BAK凋亡孔中的应用

CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions: Application of Site-Directed Spin Labeling to BAK Apoptotic Pores.

作者信息

Mandal Tirtha, Hustedt Eric J, Song Likai, Oh Kyoung Joon

机构信息

Department of Biochemistry & Molecular Biology, The Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA.

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.

出版信息

Methods Mol Biol. 2019;1877:257-303. doi: 10.1007/978-1-4939-8861-7_18.

DOI:10.1007/978-1-4939-8861-7_18

PMID:30536012

Abstract

The continuous wave (CW) and pulse electron paramagnetic resonance (EPR) methods enable the measurement of distances between spin-labeled residues in biopolymers including proteins, providing structural information. Here we describe the CW EPR deconvolution/convolution method and the four-pulse double electron-electron resonance (DEER) approach for distance determination, which were applied to elucidate the organization of the BAK apoptotic pores formed in the lipid bilayers.

摘要

连续波（CW）和脉冲电子顺磁共振（EPR）方法能够测量包括蛋白质在内的生物聚合物中自旋标记残基之间的距离，从而提供结构信息。在此，我们描述了用于距离测定的CW EPR去卷积/卷积方法和四脉冲双电子-电子共振（DEER）方法，这些方法被用于阐明脂质双层中形成的BAK凋亡孔的结构。