pafA 的转录由 PafBC 调控，pafA 编码原核泛素样蛋白连接酶。
The transcription of pafA, encoding the prokaryotic ubiquitin-like protein ligase, is regulated by PafBC.
机构信息
Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
出版信息
Future Microbiol. 2019 Jan;14:11-21. doi: 10.2217/fmb-2018-0278. Epub 2018 Dec 14.
AIM
Mycobacterium tuberculosis possesses an intracellular tagging and degradation system, which has emerged as a target for development of anti-tuberculosis agents. In this system, PafA is the ligase that marks proteins for degradation by their covalent modification with a protein modifier. Here, we studied pafA transcriptional regulation, which remained elusive despite its importance for M. tuberculosis virulence.
MATERIALS & METHODS: Working with Mycobacterium smegmatis, a mycobacterial model organism, we examined the involvement of the global regulators PafB and PafC in pafA regulation.
RESULTS
PafBC activated pafA transcription following DNA damage, resulting in efficient cellular recovery.
CONCLUSION
The results unraveled the involvement of PafBC in pafA transcription, and revealed the importance of proper PafA regulation in mycobacterial physiology.
目的
结核分枝杆菌具有一种细胞内标记和降解系统，该系统已成为开发抗结核药物的靶点。在该系统中，PafA 是一种连接酶，通过将蛋白质与蛋白质修饰剂共价修饰来标记要降解的蛋白质。在这里，我们研究了 pafA 的转录调控，尽管它对结核分枝杆菌的毒力很重要，但仍不清楚其调控机制。
材料与方法
利用分枝杆菌模型生物耻垢分枝杆菌，我们研究了全局调控因子 PafB 和 PafC 在 pafA 调控中的作用。
结果
PafBC 在 DNA 损伤后激活 pafA 转录，从而实现有效的细胞恢复。
结论
这些结果揭示了 PafBC 在 pafA 转录中的作用，并表明了适当的 PafA 调控在分枝杆菌生理学中的重要性。
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