Upadhyay Prabhat, Mishra Sunil Kumar, Mishra Awadhesh Kumar, Kumar Pradeep, Pandey Nidhi, Tiwari Kavindra Nath, Tilak Ragini, Purohit Suresh, Dubey G P
Department of Pharmacology Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.
Department of Pharmaceutical, Engineering and Technology, Indian Institute of Technology, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.
Microb Pathog. 2019 Feb;127:326-334. doi: 10.1016/j.micpath.2018.12.024. Epub 2018 Dec 12.
Reinwardtia indica (Lineceae) is a medicinal plant cultivated in the Himalayan region. It is effectively used in folk medicines for the treatment of various health complications. In the present study, the shade dried leaves and stem were extracted in three different solvents such as ethyl acetate, ethanol, and hydro-alcoholic. The antioxidant efficacy of these extracts was confirmed by using different in vitro assays: DPPH free radical scavenging, superoxide radical scavenging, lipid peroxidation, metal ion chelating capability and reducing power determination. Total phenol content was maximum in hydro alcoholic extract of leaf (540.37 mg per g of gallic acid equivalents) and stem (330.51 mg per g of gallic acid equivalents) while flavonoid content was maximum in ethanolic extract of leaf (305 mg per gram of rutin equivalents) and ethyl acetate extract of stem (170.6 mg per gram of rutin equivalents). The antioxidant activity of these extracts was positively correlated with their total phenol and flavonoid content. Among all tested extracts, ethanolic extract of leaf exhibit maximum zone of inhibition against all tested clinical isolates of bacterial (E. coli 11.00 ± 1.73 mm, P. aeurogenosa 11.67 ± 0.58 mm and S. aureus 10.33 ± 1.53 mm) and fungal (C. albicans 11.33 ± 1.10 mm) pathogens, while ethyl acetate extracts of the leaf and stem showed minimum inhibitory concentration against all tested microorganisms. Thus, R. indica extracts can be used as potent natural antioxidant having antifungal and antibacterial action.
印度翅子树（梧桐科）是一种生长在喜马拉雅地区的药用植物。它在民间医药中被有效地用于治疗各种健康问题。在本研究中，将阴干的叶子和茎用三种不同的溶剂进行提取，即乙酸乙酯、乙醇和水 - 乙醇混合溶剂。通过不同的体外试验证实了这些提取物的抗氧化功效：DPPH自由基清除、超氧阴离子自由基清除、脂质过氧化、金属离子螯合能力和还原能力测定。叶的水 - 乙醇提取物中总酚含量最高（每克没食子酸当量540.37毫克），茎的水 - 乙醇提取物中总酚含量为每克没食子酸当量330.51毫克；而叶的乙醇提取物中黄酮含量最高（每克芦丁当量305毫克），茎的乙酸乙酯提取物中黄酮含量为每克芦丁当量170.6毫克。这些提取物的抗氧化活性与其总酚和黄酮含量呈正相关。在所有测试的提取物中，叶的乙醇提取物对所有测试的临床分离细菌（大肠杆菌11.00±1.73毫米、铜绿假单胞菌11.67±0.58毫米和金黄色葡萄球菌10.33±1.53毫米）和真菌（白色念珠菌11.33±1.10毫米）病原体表现出最大的抑菌圈，而叶和茎的乙酸乙酯提取物对所有测试微生物显示出最低抑菌浓度。因此，印度翅子树提取物可作为具有抗真菌和抗菌作用的强效天然抗氧化剂。
