Jiang Haolang, Lin Lianyu, Tang Wei, Chen Xuehang, Zheng Qiaojia, Huang Jun, Yang Tao, Su Li, Dong Yanhan, Wang Baohua, Wang Zonghua
Fujian University Key Laboratory for Plant-Microbe Interaction and College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
Fujian University Key Laboratory for Plant-Microbe Interaction and College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
Evol Bioinform Online. 2018 Dec 7;14:1176934318810990. doi: 10.1177/1176934318810990. eCollection 2018.
The endoplasmic reticulum (ER) is the entry portal of the conventional secretory pathway where the newly synthesized polypeptides fold, modify, and assemble. The ER responses to the unfolded proteins in its lumen (ER stress) by triggering intracellular signal transduction pathways include the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR) pathway. In yeast and mammals, the ubiquitin ligase Hrd1 is indispensable for the ERAD pathway, and also Hrd1-mediated ERAD pathway plays a crucial role in maintaining homeostasis and metabolism of human beings. However, the underlying physiological roles and regulatory mechanism of the Hrd1-involved ERAD pathway in the plant pathogenic fungi are still unclear. Here, we identified the Hrd1 orthologous proteins from 9 different fungi and noticed that these Hrd1 orthologs are conserved. Through identification of MoHrd1 putative interacting proteins by co-immunoprecipitation assays and enrichment analysis, we found that MoHrd1 is involved in the secretory pathway, energy synthesis, and metabolism. Taken together, our results suggest that MoHrd1 is conserved among fungi and play an important role in cellular metabolism and infection-related development. Our finding helps uncover the mechanism of Hrd1-involved ERAD pathway in fungi and sheds a new light to understand the pathogenic mechanism of .
内质网（ER）是传统分泌途径的入口门户，新合成的多肽在此折叠、修饰和组装。内质网通过触发细胞内信号转导途径来应对其腔室内未折叠的蛋白质（内质网应激），这些途径包括内质网相关降解（ERAD）途径和未折叠蛋白反应（UPR）途径。在酵母和哺乳动物中，泛素连接酶Hrd1对于ERAD途径不可或缺，而且Hrd1介导的ERAD途径在维持人类的体内平衡和新陈代谢中起着关键作用。然而，在植物病原真菌中，涉及Hrd1的ERAD途径的潜在生理作用和调控机制仍不清楚。在此，我们从9种不同的真菌中鉴定出了Hrd1直系同源蛋白，并注意到这些Hrd1直系同源物是保守的。通过共免疫沉淀试验和富集分析鉴定稻瘟病菌MoHrd1的假定相互作用蛋白，我们发现MoHrd1参与分泌途径、能量合成和代谢。综上所述，我们的结果表明MoHrd1在真菌中是保守的，并且在细胞代谢和感染相关发育中起重要作用。我们的发现有助于揭示真菌中涉及Hrd1的ERAD途径的机制，并为理解……的致病机制提供了新的线索。
