Rausch U W, Fussgänger R D, Heinze E
Innere Medizin Abteilung II, Universität Ulm, Germany.
Horm Metab Res Suppl. 1988;18:89-92.
To prove hormone analogy of insulin and proinsulin 1. biochemical characterization of binding, 2. competition-inhibition-assay, 3. up-or-down-regulation, and 4. 3H-thymidine incorporation in IM 9-lymphocytes in vitro were applied. Marked differences in binding and biological activity of insulin and proinsulin in the physiological range (10 pM-1 nM) are obvious. Heterogeneity of specific insulin and proinsulin binding sites is suggested.
为证明胰岛素与胰岛素原的激素类似性，采用了以下方法：1. 结合的生化特性分析；2. 竞争抑制测定；3. 上调或下调；4. 体外IM 9淋巴细胞中3H-胸腺嘧啶核苷掺入实验。在生理范围（10皮摩尔-1纳摩尔）内，胰岛素与胰岛素原在结合及生物活性方面存在显著差异。提示存在特异性胰岛素和胰岛素原结合位点的异质性。
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