Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing 210098, China..
Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing 210098, China.
J Environ Sci (China). 2019 Mar;77:323-335. doi: 10.1016/j.jes.2018.09.004. Epub 2018 Sep 21.
Heavy metals, pharmaceuticals, and other wastes released into the environment can significantly influence environmental antibiotic resistance. We investigated the occurrence of 22 antimicrobial resistance genes (ARGs) and 10 heavy metal concentrations, and the relationship between ARGs and heavy metals in surface sediment from seven sites of Lake Taihu. The results showed significant correlations (p < 0.05) between sediment ARG levels, especially for tetracycline and sulfonamides (e.g., tet(A), tet(D), tet(E), tet(O), sul1, sul2 and int-1) and specific heavy metals (Fe, Mn, Cr, Cu, Zn, among others) in the Lake. In the surface sediments, heavy metals showed an interaction with resistance genes, but the strength of interaction was diminished with increasing depth. For most of the heavy metals, the concentration of elements in the top sediments was higher than that in other depths. Tetracycline resistance genes (tet(A), tet(B), tet(D), tet(E) and tet(O), β-lactam resistance genes (SHV, TEM, CTX, OXA and OXY) and sulfonamide resistance genes (sulA, sul1, sul2, sul3 and int-1) were detected. They showed a trend which inferred a statistically significant increase followed by decreases in the relative abundance of these ARGs (normalized to 16S rRNA genes) with increasing depth. This study revealed that tet(A), tet(O), TEM, OXY, int-1, sul1 and sul3 were widespread in surface sediments with high abundance, indicating that these genes deserve more attention in future work.
重金属、药品和其他废物排放到环境中会显著影响环境中的抗生素抗性。我们研究了 22 种抗生素抗性基因（ARGs）和 10 种重金属浓度在太湖 7 个采样点表层沉积物中的分布，并分析了 ARGs 与重金属之间的相关性。结果表明，沉积物中 ARG 水平与重金属之间存在显著相关性（p<0.05），特别是四环素类和磺胺类抗性基因（如 tet(A)、tet(D)、tet(E)、tet(O)、sul1、sul2 和 int-1）与特定重金属（Fe、Mn、Cr、Cu、Zn 等）之间存在显著相关性。在表层沉积物中，重金属与抗性基因存在相互作用，但随着深度的增加，相互作用的强度减弱。对于大多数重金属，表层沉积物中的元素浓度高于其他深度。我们检测到了四环素类抗性基因（tet(A)、tet(B)、tet(D)、tet(E)和 tet(O)）、β-内酰胺类抗性基因（SHV、TEM、CTX、OXA 和 OXY）和磺胺类抗性基因（sulA、sul1、sul2、sul3 和 int-1）。这些 ARGs 的相对丰度（归一化到 16S rRNA 基因）随着深度的增加呈现出先升高后降低的趋势。本研究表明，tet(A)、tet(O)、TEM、OXY、int-1、sul1 和 sul3 在表层沉积物中广泛存在且丰度较高，表明这些基因在未来的研究中值得更多关注。
