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两种安第斯高山垫状植物首批多态性微卫星标记的分离与交叉扩增

Isolation and cross-amplification of the first set of polymorphic microsatellite markers of two high-Andean cushion plants.

作者信息

Acuña-Rodríguez Ian S, Gouin Nicolas, Cifuentes-Lisboa Leonardo, Squeo Francisco A

机构信息

Departamento de Biología, Facultad de Ciencias, Universidad de La Serena, Av. Raúl Bitrán 1305, La Serena, Chile.

出版信息

J Genet. 2018 Oct 9;97(5):e95-e100.

Abstract

In the southern Andes mountains (27-39◦S) and , two Apiaceae cushion plant species commonly known as , conform a well-established altitudinal vegetation belt along the lower Andean zone. These species have been considered as fundamental components of several ecological dynamics within their communities; however, high mountain ecosystems are increasingly threatened worldwide by natural and anthropogenic pressures and the southern Andes are not the exception. Recognizing that genetic information is crucial for the success of any conservation or restoration initiative inwild populations, we developed and cross-amplified 28 specifically designed microsatellite markers (14 in and 14 in ), and also tested the cross amplification of 25 markers from the related species . In a region which is particularly vulnerable to global change trends, this new polymorphic microsatellite loci will be useful in the study of the genetic diversity of these high-mountain cushion plants, which are pivotal in the structuring of their native ecosystems.

摘要

在安第斯山脉南部(南纬27 - 39度),两种通常被称为……的伞形科垫状植物,沿着安第斯山脉较低区域形成了一个成熟的垂直植被带。这些物种被认为是其群落内多种生态动态的基本组成部分;然而,高山生态系统在全球范围内正日益受到自然和人为压力的威胁,安第斯山脉南部也不例外。认识到遗传信息对于野生种群任何保护或恢复计划的成功至关重要,我们开发并交叉扩增了28个专门设计的微卫星标记(……中有14个,……中有14个),还测试了来自相关物种的25个标记的交叉扩增。在一个特别容易受到全球变化趋势影响的地区,这些新的多态微卫星位点将有助于研究这些高山垫状植物的遗传多样性,它们在其原生生态系统的结构中起着关键作用。

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