Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science , Nanjing Normal University , Nanjing 210023 , People's Republic of China.
J Agric Food Chem. 2019 Jan 9;67(1):63-71. doi: 10.1021/acs.jafc.8b06154. Epub 2018 Dec 21.
Biodegradation of pesticide pollution is often restricted by environmental pressures, such as nutrient deprivation. Ensifer adhaerens CGMCC 6315 could overcome this issue and degrade neonicotinoid acetamiprid (ACE) efficiently under low nutrient stimuli. The ACE degradation rate improved by 33.1-fold when the lysogeny broth content for cell culture was decreased to 1/15-fold. Resting cells of CGMCC 6315 degraded 94.4% of 200 mg/L ACE in 12 h and quickly eliminated 87.8% of 5 mg/kg of residual soil ACE within 2 d. ACE degradation by CGMCC 6315 was via a nitrile hydratase (NHase) pathway. Genome sequencing showed that CGMCC 6315 had two NHase genes ( cnhA and pnhA). PnhA had the highest reported activity of 28.8 U/mg for ACE. QPCR and proteomic analysis showed that the improved ACE degradation ability was attributed to the up-regulated expression of PnhA. This biodegradation system of CGMCC 6315 has great potential for use in pesticide pollution remediation.
农药污染的生物降解常常受到环境压力的限制，如营养物质的缺乏。凝结芽孢杆菌 CGMCC 6315 能够克服这个问题，并在低营养刺激下有效地降解新烟碱类杀虫剂乙酰甲胺磷 (ACE)。当细胞培养用的溶菌肉汤含量降低到 1/15 时，ACE 的降解速率提高了 33.1 倍。CGMCC 6315 的休止细胞在 12 小时内降解了 200 mg/L 的 ACE 达 94.4%，并在 2 天内迅速消除了 5 mg/kg 残留土壤 ACE 的 87.8%。CGMCC 6315 通过腈水解酶 (NHase) 途径降解 ACE。基因组测序表明，CGMCC 6315 有两个 NHase 基因 (cnhA 和 pnhA)。PnhA 的 ACE 活性最高，为 28.8 U/mg。QPCR 和蛋白质组学分析表明，ACE 降解能力的提高归因于 PnhA 的上调表达。CGMCC 6315 的这种生物降解系统在农药污染修复方面具有很大的应用潜力。
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