Colella C M, Simi S, Rainaldi G, van Boxel T, Della Valle G, Peverali A F, Talarico D, Mariani T, Piras A, Simili M
Istituto di Mutagenesi e Differenziamento CNR, Pisa, Italy.
Somat Cell Mol Genet. 1988 Nov;14(6):593-604. doi: 10.1007/BF01535313.
Pseudodiploid Chinese hamster V79-AP4 cells, functionally diploid at the adenine phosphoribosyltransferase (aprt) locus, were treated with colcemid, a well-known aneuploidizing agent, under various experimental conditions. Aneuploid and tetraploid cells and variants resistant to 10 micrograms/ml of 2,6-diaminopurine (DAP), which selects for presumptive aprt+/- heterozygotes in the untreated cells, were induced. Many of the induced variants were hypotetraploid with three (rather than four) chromosomes carrying the aprt gene. Dot-blot and Southern analysis of the DNA of these clones confirmed that they had three copies of the aprt gene. Their APRT specific enzymatic activity was 60-80% of that of wild-type V79-AP4. The results of these and other experiments suggest that in these variants resistance to DAP is due to an altered aprt gene dosage and point to a possible genetic effect of colcemid and other aneuploidizing agents in somatic mammalian cells.
假二倍体中国仓鼠V79 - AP4细胞在腺嘌呤磷酸核糖转移酶（aprt）基因座功能上是二倍体，在各种实验条件下用秋水仙酰胺（一种著名的非整倍体诱导剂）处理。诱导出了非整倍体和四倍体细胞以及对10微克/毫升2,6 - 二氨基嘌呤（DAP）有抗性的变体，在未处理的细胞中，DAP可筛选出假定的aprt +/-杂合子。许多诱导出的变体是亚四倍体，带有aprt基因的染色体有三条（而非四条）。对这些克隆的DNA进行斑点印迹和Southern分析证实它们有三个aprt基因拷贝。它们的APRT特异性酶活性是野生型V79 - AP4的60 - 80%。这些实验和其他实验的结果表明，在这些变体中对DAP的抗性是由于aprt基因剂量改变，这也指出了秋水仙酰胺和其他非整倍体诱导剂在哺乳动物体细胞中可能存在的遗传效应。
