Xue Bangde, Liang Beibei, Yuan Guangyin, Zhu Limin, Wang Hao, Lu Zhaohui, Xu Zhiwei
Shanghai Children's Medical Center, National Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Department of Cardiovascular Surgery, Shanghai, China; Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Department of Cardiovascular Surgery, Shanghai, China.
Shanghai University of Medicine and Health Sciences, Shanghai, China; Biology Research Center, University of Shanghai for Science and Technology, Shanghai, China.
Int J Pediatr Otorhinolaryngol. 2019 Feb;117:88-95. doi: 10.1016/j.ijporl.2018.10.047. Epub 2018 Nov 22.
To preliminarily investigate the feasibility of bioabsorption tracheal stenting for the therapeutic application of congenital tracheal stenosis (CTS).
Experimental research.
Shanghai Children Medical Center, National Children's Medical Center.
Five kinds of magnesium alloys with different compositions were studied in this paper, a patented Mg-Nd-Zn-Zr alloy series namely JDBM (JiaoDa BioMg) and four Mg-Ca-Zn alloys. The cytotoxicity of alloys was evaluated by the MTS ([3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay in rabbit tracheal epithelial cells. Specific magnesium alloys had been immersed in the cell culture medium for 24 h. The tracheal stents that were made of magnesium alloy were implanted into the trachea of New Zealand rabbits and the ablation of the stent was monitored by fiber bronchoscopy. The routine blood examination was conducted prior to and following the stent placement. The rabbits were euthanized following 2-3 months of stenting. H&E staining of the main organs was conducted and the induction of apoptosis of the tracheal tissues was monitored.
The cytotoxicity of the JDBM magnesium alloy was mild and lower than the remaining 4 alloys. The stents were placed successfully in five animals. The tracheal stents were successfully placed and gradually biodegradated as monitored by fiber bronchoscopy; no significant systemic inflammatory response was noted. No significant differences in the liver and/or kidney function prior to and following stent placement were noted. H&E staining indicated the absence of pathological changes in the trachea, liver, heart and/or kidney tissues. The apoptotic assay indicated that the apoptosis ratio of the tracheal tissues was comparable between rabbits with and without tracheal stenting.
The results suggested the feasibility of bioabsorption stents made of biodegradable magnesium alloys using in patients with tracheal stenosis, especially in infants.
初步探讨生物吸收性气管支架用于先天性气管狭窄（CTS）治疗的可行性。
实验研究。
国家儿童医学中心上海儿童医学中心。
本文研究了五种不同成分的镁合金，一种获得专利的Mg-Nd-Zn-Zr合金系列即JDBM（交大生物镁合金）和四种Mg-Ca-Zn合金。通过MTS（[3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲氧基苯基)-2-(4-磺基苯基)-2H-四唑]）法在兔气管上皮细胞中评估合金的细胞毒性。将特定的镁合金浸入细胞培养基中24小时。将由镁合金制成的气管支架植入新西兰兔的气管，并通过纤维支气管镜监测支架的消融情况。在放置支架前后进行血常规检查。在支架置入2 - 3个月后对兔子实施安乐死。对主要器官进行苏木精-伊红（H&E）染色，并监测气管组织的凋亡诱导情况。
JDBM镁合金的细胞毒性为轻度，低于其余4种合金。支架成功植入5只动物体内。通过纤维支气管镜监测发现气管支架成功置入并逐渐生物降解；未观察到明显的全身炎症反应。在放置支架前后，肝和/或肾功能无显著差异。H&E染色表明气管、肝脏、心脏和/或肾脏组织无病理变化。凋亡检测表明，气管支架置入组和未置入组兔子气管组织的凋亡率相当。
结果表明，由可生物降解镁合金制成的生物吸收性支架用于气管狭窄患者，尤其是婴儿是可行的。
Zotero 插件