基于 SERS 的新型适体传感器用于微囊藻毒素-LR 的超灵敏检测。
A novel SERS-based aptasensor for ultrasensitive sensing of microcystin-LR.
机构信息
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
出版信息
Food Chem. 2019 Apr 25;278:197-202. doi: 10.1016/j.foodchem.2018.11.071. Epub 2018 Nov 13.
We developed a novel aptasensor based on surface enhanced Raman spectroscopy (SERS) and applied it for highly sensitive detection of microcystin-LR (MC-LR). In this work, MC-LR aptamer and its corresponding complementary DNA fragments (cDNA) were conjugated to gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs), respectively. Then, MC-LR aptamer-AuNPs and cDNA-MNPs conjugates were used as signal probes and capture probes, respectively. The proposed assay exhibited a linearity range from 0.01 to 200 ng/mL with the limit of detection (LOD) of 0.002 ng/mL. In addition, the reliability of the novel approach was validated by detecting different levels of MC-LR spiked in tap water samples. Overall, the novel aptasensor paves a new way for rapid and accurate determination of MC-LR and can be referred to detect other hazardous substances in water products.
我们开发了一种基于表面增强拉曼光谱（SERS）的新型适体传感器，并将其应用于微囊藻毒素-LR（MC-LR）的高灵敏检测。在这项工作中，MC-LR 适体及其相应的互补 DNA 片段（cDNA）分别与金纳米粒子（AuNPs）和磁性纳米粒子（MNPs）偶联。然后，将 MC-LR 适体-AuNPs 和 cDNA-MNPs 缀合物分别用作信号探针和捕获探针。所提出的测定方法表现出从 0.01 到 200ng/mL 的线性范围，检测限（LOD）为 0.002ng/mL。此外，通过检测自来水中不同水平的 MC-LR 加标样品，验证了新方法的可靠性。总的来说，新型适体传感器为 MC-LR 的快速准确测定开辟了新途径，并可用于检测水产品中的其他有害物质。
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