Salimi Mojtaba, Rassi Yavar, Chatrabgoun Omid, Kamali Artin, Oshaghi Mohammad Ali, Shiri-Ghaleh Vida, Moradi Mehrdad, Rafizadeh Sayena, Akbarzadeh Kameran, Parkhideh Seyedeh Zahra
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Statistics, Faculty of Mathematical Science and Statistics, Malayer University, Malayer, Iran.
J Arthropod Borne Dis. 2018 Sep 30;12(3):219-231. eCollection 2018 Sep.
Entomotoxicology as a subset of forensic entomology can be used by analysis of carcass feeding insects to detecting of drugs or toxins, as well as the cause and manner of death in cases of ante-mortem drugs intoxication. Morphine is one of the deacetylate metabolites of heroin. The aim of this study was to determine the presence and quantity of morphine in insects on the carcass and compare them with decomposing carcass.
Field of this study was in Chalabeh District and toxicological tests were carried out at the Department of Forensic Toxicology, Legal Medicine Center, Kermanshah, Iran in 2017. Morphine was inoculated into live rabbit as experimental model at concentrations of 12.5, 25, 50mg/ml, similar to those normally encountered in human overdoses, then quality and quantity of morphine were determined in insects such as (as the first wave of insect succession on human cadavers) fed on carcass.
Quantitative assessment at larvae showed that morphine was detected in all larvae (feeding and post feeding stage) fed on tissues from carcasses administered morphine, except for post-feeding larvae from R1 which received 12.5mg/ml dosage of morphine.
Necrophagous insects are an indicator on the scene of crime and a potential source of information about the antemortem situation. Detection of drug in insects which is actually a reflection of the cause of death is possible.
昆虫毒理学作为法医昆虫学的一个分支,可通过对尸体取食昆虫的分析来检测药物或毒素,以及在生前药物中毒案件中的死因和死亡方式。吗啡是海洛因的脱乙酰代谢产物之一。本研究的目的是确定尸体上昆虫体内吗啡的存在情况和含量,并将其与正在腐烂的尸体进行比较。
本研究的地点在查拉贝区,毒理学测试于2017年在伊朗克尔曼沙赫法医学中心法医毒理学部进行。将吗啡以12.5、25、50mg/ml的浓度接种到活兔体内作为实验模型,这些浓度与人类过量用药时通常遇到的浓度相似,然后测定取食尸体的昆虫(如作为人类尸体上昆虫演替的第一波)体内吗啡的质量和含量。
对幼虫的定量评估表明,在取食给予吗啡的尸体组织的所有幼虫(取食阶段和取食后阶段)中均检测到吗啡,但接受12.5mg/ml吗啡剂量的R1组取食后幼虫除外。
食尸性昆虫是犯罪现场的一个指标,也是有关生前情况的潜在信息来源。在昆虫体内检测药物实际上反映了死因,这是可能的。