Dougan G, Chatfield S, Pickard D, Bester J, O'Callaghan D, Maskell D
Department of Molecular Biology, Wellcome Research Laboratories, Beckenham, Kent, England.
J Infect Dis. 1988 Dec;158(6):1329-35. doi: 10.1093/infdis/158.6.1329.
Derivatives of the mouse-virulent Salmonella typhimurium strain SL1344 were constructed harboring stable mutations in aroC alone or in aroC and aroA together. Fifty percent lethal doses after intravenous inoculation of the mutants into BALB/c mice were determined, and the mutants were as highly attenuated as were SL1344 aroA derivatives. All aro-dependent derivatives persisted in vivo at similar levels and for similar intervals in the livers and spleens of BALB/c mice infected intravenously. Mice vaccinated orally with 10(10) live organisms of the different derivatives survived and were well protected against oral challenge with virulent S. typhimurium. A Salmonella typhi Ty2 derivative, designated WBL2000, was constructed that harbors well-defined, stable mutations in both aroA and aroC.
构建了鼠毒力鼠伤寒沙门氏菌菌株SL1344的衍生物，其aroC单独或aroC和aroA一起发生稳定突变。将突变体静脉内接种到BALB/c小鼠后测定50%致死剂量，这些突变体与SL1344 aroA衍生物一样高度减毒。所有aro依赖性衍生物在静脉内感染的BALB/c小鼠的肝脏和脾脏中以相似水平和相似间隔在体内持续存在。用不同衍生物的10(10)个活生物体口服免疫的小鼠存活下来，并得到很好的保护，免受毒力鼠伤寒沙门氏菌的口服攻击。构建了一种伤寒沙门氏菌Ty2衍生物，命名为WBL2000，其aroA和aroC均发生了明确的稳定突变。
