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[干燥方法对平菇食品特性及降血脂潜力的影响]

[The influence of the drying method for food properties and hypolidemic potential of oyster mushrooms ].

作者信息

Piskov S I, Timchenko L D, Rzhepakovsky I V, Avanesyan S S, Sizonenko M N, Areshidze D A, Kovalev D A

机构信息

North-Caucasus Federal University, Stavropol.

Moscow Region State University, Moscow.

出版信息

Vopr Pitan. 2018;87(2):65-76. doi: 10.24411/0042-8833-2018-10020. Epub 2018 Feb 26.

Abstract

The aim of this research was to study the influence of two drying methods: freeze-drying sublimation and dry-air drying on the selected nutritional properties and hypolipidemic potential of fruiting bodies of oyster mushroom (Pleurotus ostreatus). The criteria for evaluation of the food properties were the color, the morphological structure, regidratation capacity, the total level of soluble proteins, fats, polysaccharides, free amino acids and monosaccharides. Lipid-lowering potential of oyster mushroom was evaluated by the concentration of lovastatin and the level of antioxidant activity. It has been experimentally revealed that the value of optical density of hydro-alcohol extracts of dried oyster mushrooms at a wavelength of 295 nm most clearly characterized its color which intensity was almost twice less in sublimated mushrooms, than шт the sample dried by dry-air method. Histological data showed that dry-air drying lead to the destruction of the mushroom cells and to the formation of a dense layered structure. Sublimation drying preserved the ordered cell structure and provided less deformation and shrinkage of the tissues. Using X-ray microtomography it was reported that freeze-dried mushrooms had uniform pore volume distribution. Dry-air dehydration method lead to the formation of larger cavities. The average percentage of the open pores was: 29.41±0.52% (after dry-air method), 11.10±0.41% (after freeze-drying method). Respectively the number of closed pores, which reflected the true value of porosity, was 0.99±0.01 and 1.75±0.01%. Structural differences of the samples of the dry oyster mushroom combined with their unequal hydration ability. Indicator of rehydration for oyster mushroom dried by sublimation method was 5.4±0.1, and for samples obtained by dry-air method it was 3.2±0.1. Respectively the average time of maximum water absorption was 22.7±1.8 and 45.3±2.9 minutes. It was found that the freeze-drying sublimation conditions were more conducive for the preservation of the biologically active protein and polysaccharide components of oyster mushrooms and on the other hand dry-air drying method increased the nutritional value of oyster mushrooms due to the reactions of polysaccharides autohydrolysis. The number of proteins and polysaccharides of the Oyster mushrooms samples dried by dry-air method and freeze-drying method was 72.0% and 56.0% respectively. Concentrations of free amino acids and glucose in the samples dried by freeze-drying and dry-air methods were 11.60±0.31%; 175.20±6.10 mg% and 7.00±0.28%; 144.0±5.7 mg% respectively. It has been experimentally recorded that the conditions of freeze drying were optimal in terms of ensuring the preservation of the content of natural statin and the antioxidant capacity of oyster mushrooms that provided its hypolipidemic potential. The amount of lovastatin in an the freeze-dried samples was 342±9.0 mg/kg, and was significantly higher than in the samples received by dry-air method - 190±6.0 mg/kg. The level of antioxidant activity of the oyster mushrooms samples were respectively 3.83±0.02 against 2.0±0.03 mmol/100 g. The conducted researches proved that for the production of dry oyster mushroom as a potential biologically active feedstock for the functional food products with lipid-regulating directivity the choice of the drying method had a fundamental importance.

摘要

本研究的目的是研究两种干燥方法

冷冻干燥升华法和热风干燥法对平菇(糙皮侧耳)子实体所选营养特性和降血脂潜力的影响。评估食品特性的标准包括颜色、形态结构、复水能力、可溶性蛋白质、脂肪、多糖、游离氨基酸和单糖的总量。通过洛伐他汀浓度和抗氧化活性水平评估平菇的降脂潜力。实验表明,干平菇水醇提取物在295nm波长处的光密度值最能清晰地表征其颜色,升华干燥的平菇颜色强度几乎比热风干燥法干燥的样品低两倍。组织学数据表明,热风干燥导致蘑菇细胞破坏并形成致密的层状结构。升华干燥保留了有序的细胞结构,使组织变形和收缩较小。利用X射线显微断层扫描技术发现,冷冻干燥的蘑菇具有均匀的孔隙体积分布。热风脱水法导致形成更大的空洞。开孔的平均百分比为:29.41±0.52%(热风干燥法后),11.10±0.41%(冷冻干燥法后)。相应地,反映孔隙率真实值的闭孔数量分别为0.99±0.01%和1.75±0.01%。干平菇样品的结构差异与其不等的水合能力相结合。升华法干燥平菇的复水指标为5.4±0.1,热风干燥法得到的样品为3.2±0.1。相应地,最大吸水平均时间分别为22.7±1.8分钟和45.3±2.9分钟。研究发现,冷冻干燥升华条件更有利于平菇生物活性蛋白质和多糖成分的保存,另一方面,热风干燥法由于多糖自水解反应提高了平菇的营养价值。热风干燥法和平菇冷冻干燥法样品中蛋白质和多糖的含量分别为72.0%和56.0%。冷冻干燥法和热风干燥法样品中游离氨基酸和葡萄糖浓度分别为11.60±0.31%;175.20±6.10mg%和7.00±0.28%;144.0±5.7mg%。实验记录表明,就确保平菇中天然他汀含量的保存及其提供降血脂潜力的抗氧化能力而言,冷冻干燥条件是最佳的。冷冻干燥样品中洛伐他汀的含量为342±9.0mg/kg,显著高于热风干燥法得到的样品——190±6.0mg/kg。平菇样品的抗氧化活性水平分别为3.83±0.02和2.0±0.03mmol/100g。所进行的研究证明,对于生产作为具有脂质调节导向功能的功能性食品潜在生物活性原料的干平菇,干燥方法的选择具有根本重要性。

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