使用培养的丙型肝炎病毒进行体外中和试验。

In Vitro Neutralization Assay Using Cultured Hepatitis C Virus.

作者信息

Prentoe Jannick, Bukh Jens

机构信息

Copenhagen Hepatitis C Program (CO-HEP), Department of Infectious Diseases, Hvidovre Hospital, Hvidovre, Denmark.

Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

出版信息

Methods Mol Biol. 2019;1911:433-439. doi: 10.1007/978-1-4939-8976-8_29.

DOI:10.1007/978-1-4939-8976-8_29

PMID:30593643

Abstract

The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.

摘要

本文概述的方法能够评估单克隆抗体和多克隆抗体针对体外培养的丙型肝炎病毒（HCV）的中和效力。HCV分离株之间包膜蛋白序列的高度变异性使得有必要纳入几种涵盖HCV主要基因型的分离株，以便就给定抗体的交叉反应中和潜力得出有力结论。这对于任何旨在发现新型治疗性或疫苗相关抗体的中和实验尤为重要。此外，这些检测方法还可用于比较新型培养的HCV与先前鉴定的分离株的中和敏感性。