Laboratoire Analyses, Valorisation et Sécurité des Aliments, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, Route Soukra, Sfax 3038, Tunisia.
Univ Lyon, Université Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA, Equipe Mixte d'Accueil n°3733, rue Henri de Boissieu, F-01000 Bourg en Bresse, France.
Int J Biol Macromol. 2019 May 1;128:347-353. doi: 10.1016/j.ijbiomac.2018.12.244. Epub 2018 Dec 27.
The influence of the addition of low methoxyl amidated pectin (LMA) on acid milk gels from whole camel milk (WCM) on physicochemical and rheological proprieties were studied. The zeta potential, particle size, viscosity, dynamic oscillatory rheology and isothermal titration calorimetry were monitored. The presence of LM-pectin in milk had an impact on the average size of the casein micelles and a large and dominant influence on rheological behavior during acidification. Zeta potential and viscosity of gels with 0.5% pectin were not affected. However, milk gels containing 1%, 1.5% and 2% of LM-pectin showed highest values of particle size at pH4. This modification of the structure of the casein micelles induces a significant improvement (p<0.05) on its acid gelation behavior. Therefore, the addition of pectin enhanced the rheological proprieties. Higher pectin concentration led to a strong gel with higher G' values. This result could be attributed to the formation of complexes and the mechanical spectra prove the hypothesis that pectin forms strands with caseins micelles. Isothermal titration calorimetry results showed that pectin concentration had a marked influence on the gels structure and that this polysaccharide stabilizes caseins micelles in acidified camel milk gel due to electrostatic interaction and steric repulsion.
研究了低甲氧基酯化果胶(LMA)的添加对全骆驼乳(WCM)酸乳凝胶的物理化学和流变性质的影响。监测了 Zeta 电位、粒径、粘度、动态振荡流变学和等温滴定量热法。乳中存在 LM-果胶会影响酪蛋白胶束的平均粒径,并对酸化过程中的流变行为产生很大的影响。含 0.5%果胶的凝胶的 Zeta 电位和粘度不受影响。然而,含 1%、1.5%和 2%LM-果胶的牛奶凝胶在 pH4 时表现出最大的粒径。这种对酪蛋白胶束结构的修饰显著改善(p<0.05)了其酸凝胶化行为。因此,果胶的添加增强了流变性能。较高的果胶浓度导致具有更高 G'值的强凝胶。这一结果可以归因于复合物的形成,机械光谱证明了果胶与酪蛋白胶束形成链的假设。等温滴定量热法结果表明,果胶浓度对凝胶结构有显著影响,由于静电相互作用和空间排斥,这种多糖稳定了酸化骆驼乳凝胶中的酪蛋白胶束。