衰老对大鼠骨骼肌和白色脂肪组织中 Rho 激酶和胰岛素信号的影响。
The Effects of Aging on Rho-Kinase and Insulin Signaling in Skeletal Muscle and White Adipose Tissue of Rats.
机构信息
Laboratory of Molecular Biology of Exercise, University of Campinas (UNICAMP), Limeira, São Paulo, Brazil.
Laboratory of Functional Properties in Foods, School of Applied Sciences (FCA), University of Campinas (UNICAMP), Limeira, São Paulo, Brazil.
出版信息
J Gerontol A Biol Sci Med Sci. 2020 Feb 14;75(3):432-436. doi: 10.1093/gerona/gly293.
The insulin receptor substrate 1 regulates insulin-mediated glucose uptake and is a target of Rho-kinase (Rock); however, the relationship between age-related insulin resistance and Rock signaling specifically in skeletal muscle and adipose tissue is unknown. We evaluated the content and activity of Rock in C2C12 myotubes, and in skeletal muscle and white adipose tissue (WAT) from two rodent models that differ in their patterns of body fat accumulation during aging (Wistar and Fischer 344 rats). Body fat gain in the Wistar rats was greater than in Fischer rats and only Wistar rats had impairment of whole-body insulin sensitivity. Rock activity and insulin signaling were impaired in skeletal muscle in both rat models, but only middle-aged Wistar rats had higher Rock activity in WAT. These data are consistent with a positive role of Rock in regulating insulin signaling in both skeletal muscle and its negative role in the adipose tissue, suggesting that Rock activity in adipose tissue is important in age-related insulin resistance.
胰岛素受体底物 1 调节胰岛素介导的葡萄糖摄取，是 Rho-激酶（Rock）的靶标；然而，与年龄相关的胰岛素抵抗以及骨骼肌和脂肪组织中 Rock 信号的关系尚不清楚。我们评估了 C2C12 肌管以及两种在衰老过程中体脂积累模式不同的啮齿动物模型（Wistar 和 Fischer 344 大鼠）的骨骼肌和白色脂肪组织（WAT）中 Rock 的含量和活性。Wistar 大鼠的体脂增加量大于 Fischer 大鼠，只有 Wistar 大鼠的全身胰岛素敏感性受损。两种大鼠模型的骨骼肌中 Rock 活性和胰岛素信号均受损，但只有中年 Wistar 大鼠的 WAT 中 Rock 活性更高。这些数据表明 Rock 在调节骨骼肌中胰岛素信号方面具有积极作用，而在脂肪组织中具有消极作用，提示脂肪组织中 Rock 活性在与年龄相关的胰岛素抵抗中很重要。
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