Asnani P J, Bhatnagar N, Bhandari S
Department of Microbiology, Panjab University, Chandigarh, India.
Folia Microbiol (Praha). 1988;33(5):393-400. doi: 10.1007/BF02925850.
Eight strains of Escherichia coli, isolated from patients with a urinary tract infection were investigated for production of hemolysin. Six of these produced hemolysin and one revealed maximum hemolytic activity. Three urinary and two faecal isolates were positive for mannose-resistant hemagglutination. One isolate positive for hemagglutination and giving maximum hemolytic activity was then used. Hemolysin was present in the supernatant broth and the medium of choice to obtain the optimum yield was the alkaline meat extract broth followed by brain heart infusion broth. The highest yield appeared in the exponential phase of growth. Hemolysin is a heat-labile protein, being produced optimally at pH 8. A three-stage procedure was the best method for its purification.
对从尿路感染患者中分离出的8株大肠杆菌进行了溶血素产生情况的研究。其中6株产生溶血素，1株显示出最大溶血活性。3株尿液分离株和2株粪便分离株对甘露糖抗性血凝反应呈阳性。然后使用1株血凝反应呈阳性且具有最大溶血活性的分离株。溶血素存在于上清液中，获得最佳产量的首选培养基是碱性肉浸液肉汤，其次是脑心浸液肉汤。最高产量出现在生长的指数期。溶血素是一种热不稳定蛋白，在pH 8时产生最佳。三步法是其纯化的最佳方法。
