Synthetic and Natural Functional Molecule Chemistry of Ministry of Education Key Laboratory, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi 710127, PR China.
Synthetic and Natural Functional Molecule Chemistry of Ministry of Education Key Laboratory, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi 710127, PR China.
Talanta. 2019 Mar 1;194:548-553. doi: 10.1016/j.talanta.2018.10.057. Epub 2018 Oct 21.
Two labeled and non-label electrochemical peptide-based biosensors for highly sensitive and selective determination of hemopexin domain of matrix metalloproteinase-14 (PEX-14) were reported for the first time. Herein, the thiolated PEX-14 binding peptide inhibitor (ISC) or the ISC-tagged with ferrocene carboxylic acid (CIS-Fc) was self-assembled on the surface of gold electrode tested for binding with PEX-14 by non-label electrochemical impedance spectroscopy or labeled electrochemical (L-EC) transducers. The two biosensors were compared for the analytical performance of the detection of PEX-14 with detection limit of 0.3 pg mL (for L-EC biosensor) and 0.03 ng mL (for EIS biosensor). This work demonstrates that probing the PEX of MMPs represents a novel approach to assess MMPs-mediated cancer dissemination and provide a platform for screening the inhibitors of Matrix metalloproteinases.
两种标记和非标记的基于肽的电化学生物传感器，用于高度灵敏和选择性地测定基质金属蛋白酶-14（MMP-14）的血红素结合蛋白结构域（PEX-14），这在以前是从未有过的。在此，巯基化的 PEX-14 结合肽抑制剂（ISC）或与二茂铁羧酸标记的 ISC（CIS-Fc）通过非标记电化学阻抗谱或标记电化学（L-EC）换能器自组装在金电极表面，以测试与 PEX-14 的结合。比较了两种生物传感器在检测 PEX-14 方面的分析性能，检测限分别为 0.3pgmL（L-EC 生物传感器）和 0.03ngmL（EIS 生物传感器）。这项工作表明，探测 MMPs 的 PEX 代表了一种评估 MMPs 介导的癌症扩散的新方法，并为筛选基质金属蛋白酶抑制剂提供了一个平台。
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