用巯基处理血清或血浆以降低IgM抗体。

Sulfhydryl treatment of serum or plasma for the reduction of IgM antibodies.

作者信息

Blagg Lorraine N

机构信息

Education and Development Coordinator, Transfusion Medicine Division, The Johns Hopkins Hospital.

出版信息

Immunohematology. 2018 Dec;34(4):135-139.

PMID:30624946

Abstract

Dithiothreitol (DTT) and 2-mercaptoethanol (2-ME) are sulfhydryl compounds that can be used to treat serum or plasma to denature IgM antibody reactivity. By using sulfhydryl agents, IgG and IgM antibodies can be separated, the relative amount of IgM and IgG antibodies can be determined, and the risk of hemolytic disease of the fetus and newborn can be assessed.

摘要

二硫苏糖醇（DTT）和2-巯基乙醇（2-ME）是巯基化合物，可用于处理血清或血浆以使其IgM抗体反应性变性。通过使用巯基试剂，可以分离IgG和IgM抗体，确定IgM和IgG抗体的相对量，并评估胎儿和新生儿溶血病的风险。