Mahgoub H A M, Eisa G S A, Youssef M A H
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Agriculture Botany Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
J Genet Eng Biotechnol. 2015 Jun;13(1):39-49. doi: 10.1016/j.jgeb.2014.11.004. Epub 2014 Dec 20.
Potato ( L.) is widely used for many industrial and food applications. Nine potato cultivars were planted and collected from a private farm in new Salihiyyah city, Sharkia governorate, Egypt to compare between them at morphological, molecular, biochemical and anatomical levels. Our results indicated that the Inova cultivar was better, however the Bafana cultivar was worse in relation to yield parameters. Inter simple sequence repeat (ISSR) molecular marker has been used to determine the genetic diversity between these nine cultivars. Through using ten primers we obtained 98 bands, 85 of which were polymorphic by 87%. The highest similarity value (0.827) was found between Caruso and Alliance as the closest but the lowest value (0.418) was found between Charlotte and Bafana as the most distant. Everest tuber contained great amounts of total phenolic and peroxidase activity, while the Bafana tuber contained small amounts of it compared to other cultivars. The phellem layer of the Everest tuber had more thickness than others and the number of phellem rows was the highest. However, the Bafana cultivar listed the lowest value compared to other cultivars. Lower values from both of total bacterial and total fungi were recorded on the tuber of the Everest cultivar. However, Bafana cultivar was recorded to have a higher value of both compared to other cultivars. We suggest that the ISSR marker is a suitable procedure to examine the potato's genetic diversity at the DNA level. The Everest cultivar is considering the best cultivar to planting and breeding in Egypt.
马铃薯（L.）广泛应用于许多工业和食品领域。从埃及谢赫村省新萨利希亚市的一个私人农场种植并收集了9个马铃薯品种，以便在形态、分子、生化和解剖水平上对它们进行比较。我们的结果表明，就产量参数而言，Inova品种表现较好，而Bafana品种较差。简单序列重复区间（ISSR）分子标记已被用于确定这9个品种之间的遗传多样性。通过使用10种引物，我们获得了98条带，其中85条具有多态性，多态性比例为87%。发现Caruso和Alliance之间的相似性值最高（0.827），它们最为接近；而Charlotte和Bafana之间的相似性值最低（0.418），它们最为疏远。珠穆朗玛峰品种的块茎含有大量的总酚和过氧化物酶活性，而与其他品种相比，Bafana品种的块茎含量较少。珠穆朗玛峰品种块茎的木栓层比其他品种更厚，木栓行数也最多。然而，与其他品种相比，Bafana品种的值最低。珠穆朗玛峰品种块茎上记录的总细菌和总真菌数量均较低。然而，与其他品种相比，记录显示Bafana品种在这两者上的值较高。我们认为ISSR标记是在DNA水平上检测马铃薯遗传多样性的合适方法。珠穆朗玛峰品种被认为是埃及种植和育种的最佳品种。
