Department of Medicine III, University Hospital RWTH Aachen, Aachen, Germany.
Department of Visceral and Transplantation Surgery, University Hospital RWTH Aachen, Aachen Germany.
PLoS One. 2019 Jan 17;14(1):e0210944. doi: 10.1371/journal.pone.0210944. eCollection 2019.
Cholangiocarcinoma (CCA) represents the second most common primary hepatic malignancy. Despite tremendous research activities, the prognosis for the majority of patients is still poor. Only in case of early diagnosis, liver resection might potentially lead to long-term survival. However, it is still unclear which patients benefit most from extensive liver surgery, highlighting the need for new diagnostic and prognostic stratification strategies.
Serum concentrations of a 4 miRNA panel (miR-122, miR-192, miR-29b and miR-155) were analyzed using semi-quantitative reverse-transcriptase PCR in serum samples from 94 patients with cholangiocarcinoma undergoing tumour resection and 40 healthy controls. Results were correlated with clinical data.
Serum concentrations of miR-122, miR-192, miR-29b and miR-155 were significantly elevated in patients with CCA compared to healthy controls or patients with primary sclerosing cholangitis without malignant transformation. Although preoperative levels of these miRNAs were unsuitable as a prognostic marker of survival, a strong postoperative decline of miR-122 serum levels was significantly associated with a favorable patients' prognosis.
Analysis of circulating miRNAs represents a promising tool for the diagnosis of even early stage CCA. A postoperative decline in miRNA serum concentrations might be indicative for a favorable patients' outcome and helpful to identify patients with a good prognosis after extended liver surgery.
胆管癌（CCA）是第二大常见的原发性肝恶性肿瘤。尽管进行了大量的研究，但大多数患者的预后仍然很差。只有在早期诊断的情况下，肝切除术才有可能导致长期生存。然而，目前仍不清楚哪些患者从广泛的肝手术中获益最大，这凸显了需要新的诊断和预后分层策略。
使用半定量逆转录 PCR 分析 94 例接受肿瘤切除术的胆管癌患者和 40 例健康对照者血清样本中 4 种 miRNA （miR-122、miR-192、miR-29b 和 miR-155）的血清浓度。结果与临床数据相关联。
与健康对照组或原发性硬化性胆管炎无恶性转化的患者相比，CCA 患者的血清 miR-122、miR-192、miR-29b 和 miR-155 浓度显著升高。尽管这些 miRNA 的术前水平不适合作为生存的预后标志物，但 miR-122 血清水平的强烈术后下降与患者的良好预后显著相关。
循环 miRNA 的分析是诊断早期 CCA 的一种很有前途的工具。miRNA 血清浓度的术后下降可能提示患者预后良好，并有助于识别接受广泛肝手术后预后良好的患者。
