玫瑰黄素产生菌达沃链霉菌培养条件及基本分子工具的优化

OPTIMIZATION OF THE CULTIVATION CONDITIONS AND THE BASIC MOLECULAR TOOLS FOR ROSEOFLAVIN PRODUCER STREPTOMYCES DAVAWENSIS.

作者信息

Tsyrulnyk A O, Kordiaka R, Dmytruk K V, Fedorovych D V, Sybirny A A

出版信息

Mikrobiol Z. 2016 Jul;78(4):2-10.

Abstract

Roseoflavin (RoF), and its metabolic precursor 8-dimethylaminoriboflavin (AF), produced by Gram-positive bacteria Streptomyces davawensis and Streptomyces cinnabarinus, reveal a strong antibiotic effect against Staphyloccus aureus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus and other Gram-positive bacteria and could be promising for developing the new effective antibacterial drugs. In this work, we present the optimization of basic molecular tools for S. davawensis including total DNA preparation, PCR amplification of target gene, cell transformation. In addition, the compositions of cultivation media were studied and the optimal cultivation conditions for increased of RoF production have been developed.

摘要

由革兰氏阳性细菌达瓦链霉菌和朱红链霉菌产生的玫红菌素（RoF）及其代谢前体8-二甲基氨基核黄素（AF），对金黄色葡萄球菌、枯草芽孢杆菌、蜡样芽孢杆菌、藤黄微球菌和其他革兰氏阳性细菌显示出强大的抗菌作用，有望用于开发新型有效的抗菌药物。在这项工作中，我们展示了达瓦链霉菌基础分子工具的优化，包括总DNA制备、目标基因的PCR扩增、细胞转化。此外，还研究了培养基的成分，并制定了提高RoF产量的最佳培养条件。