Hori T, Kashiyama S, Oku N, Hayakawa M, Shibamoto S, Tsujimoto M, Nishihara T, Ito F
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Cell Struct Funct. 1988 Oct;13(5):425-33. doi: 10.1247/csf.13.425.
Human recombinant tumor necrosis factor (TNF) stimulated the growth of confluent human fibroblasts (FS-4) in the presence of fetal calf serum. Epidermal growth factor (EGF) similarly stimulated cellular growth; however other mitogenic factors such as insulin, fibroblast growth factor, 12-O-tetradecanoyl-phorbol-12-acetate and Ca2+ ionophore A23187 did not. The growth-stimulating action of TNF was not synergistic with the activity of EGF in the presence of serum. TNF induced a rapid increase in the binding of transferrin to the cell surface, followed by a return to the basal level within 5 min. A similar increase in transferrin binding was observed in FS-4 cells exposed to EGF. In contrast, insulin caused a prolonged stimulation of transferrin binding. These results suggest that TNF and EGF generate similar or identical intracellular signals for cellular growth and the regulation of transferrin receptor expression.
在胎牛血清存在的情况下，人重组肿瘤坏死因子（TNF）刺激汇合的人成纤维细胞（FS-4）生长。表皮生长因子（EGF）同样刺激细胞生长；然而，其他促有丝分裂因子，如胰岛素、成纤维细胞生长因子、12-O-十四烷酰佛波醇-12-乙酸酯和Ca2+离子载体A23187则没有这种作用。在血清存在的情况下，TNF的生长刺激作用与EGF的活性没有协同作用。TNF诱导转铁蛋白与细胞表面的结合迅速增加，随后在5分钟内恢复到基础水平。在暴露于EGF的FS-4细胞中也观察到转铁蛋白结合的类似增加。相比之下，胰岛素导致转铁蛋白结合的持续刺激。这些结果表明，TNF和EGF产生相似或相同的细胞内信号用于细胞生长和转铁蛋白受体表达的调节。
