脂双层中的MCU调节与电生理记录

MCU Regulation in Lipid Bilayer and Electrophysiological Recording.

作者信息

Checchetto Vanessa, Szabò Ildikò

机构信息

Department of Biology, University of Padua, Padua, Italy.

出版信息

Methods Mol Biol. 2019;1925:59-63. doi: 10.1007/978-1-4939-9018-4_5.

DOI:10.1007/978-1-4939-9018-4_5

PMID:30674016

Abstract

The mitochondrial calcium uniporter (MCU) and the mitochondrial calcium uniporter dominant negative b- subunit (MCUb) are pore-forming components of the uniporter complex. We expressed these MCU subunits in cell-free transcription/translation systems, and we studied them, at the single molecule level, using the electrophysiological technique of planar lipid bilayer.We showed that MCU gives rise to single-channel Ca currents. In contrast, MCUb alone does not display calcium-permeable channel activity, while the co-expression of MCUb:MCU drastically alters the calcium permeation mediated by MCU subunit.

摘要

线粒体钙单向转运体（MCU）和线粒体钙单向转运体显性负性β亚基（MCUb）是单向转运体复合物的成孔成分。我们在无细胞转录/翻译系统中表达了这些MCU亚基，并使用平面脂质双层电生理技术在单分子水平上对它们进行了研究。我们发现MCU会产生单通道钙电流。相比之下，单独的MCUb不显示钙通透通道活性，而MCUb与MCU共表达则会显著改变由MCU亚基介导的钙渗透。