Johnson Martin
Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA, USA.
Methods Mol Biol. 2019;1925:163-172. doi: 10.1007/978-1-4939-9018-4_15.
The store-operated calcium (Ca) entry (SOCE) pathway is an essential Ca signaling pathway in non-excitable cells that serve many physiological functions. SOCE is mediated through the plasma membrane (PM) protein, Orai1, and the endoplasmic reticulum protein, stromal interaction molecule 1 (STIM1). One of the most well-established methods to study SOCE is using the Ca-sensing dye, fura-2. Here we describe a detailed protocol on how to use fura-2 to study Ca signaling from SOCE in human embryonic kidney (HEK) cells.
store-operated calcium (Ca) entry (SOCE)途径是一种在非兴奋性细胞中至关重要的Ca信号通路，这些细胞具有多种生理功能。SOCE是通过质膜（PM）蛋白Orai1和内质网蛋白基质相互作用分子1（STIM1）介导的。研究SOCE最成熟的方法之一是使用Ca传感染料fura-2。在这里，我们描述了一个详细的方案，说明如何使用fura-2来研究人胚肾（HEK）细胞中SOCE的Ca信号。
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