自噬通量的阻断与 B 细胞慢性淋巴细胞白血病中的淋巴细胞增多和更高比例的肿瘤细胞有关。
Blockage of autophagic flux is associated with lymphocytosis and higher percentage of tumoral cells in chronic lymphocytic leukemia of B cells.
机构信息
Servicio de Hematología, Complejo Hospitalario Universitario de Albacete, 02006, Albacete, Spain.
Laboratorio de Oncología Molecular, Unidad de Medicina Molecular, Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, 02006, Albacete, Spain.
出版信息
Clin Transl Oncol. 2019 Sep;21(9):1280-1285. doi: 10.1007/s12094-019-02041-x. Epub 2019 Jan 24.
PURPOSE
Autophagy has lately emerged as an important biological process with implications in several hematological pathologies. Recently, a growing body of evidence supports a putative role of autophagy in chronic lymphocytic leukemia; however, no definitive clue has been established so far. To elucidate this issue, we have developed a pilot study to measure autophagic flux in peripheral blood mononuclear cells from chronic lymphocytic leukemia patients, and explored its correlation with classical clinical/analytical parameters.
METHODS/PATIENTS: Thirty-three chronic lymphocytic leukemia patients participated in the study. Autophagic flux in peripheral blood mononuclear cells was determined by western blot measuring the levels of the proteins p62 and lipidated LC3. Moreover, p62 mRNA levels were analyzed by RT-qPCR.
RESULTS
Lymphocytosis and the percentage of tumoral lymphocytes in chronic lymphocytic leukemia patients statistically correlate with a blocked autophagic flux.
CONCLUSION
Alterations in autophagic flux could play an important role in the physiopathology of chronic lymphocytic leukemia.
目的
自噬最近成为一个具有重要生物学意义的过程，与几种血液病理学相关。最近，越来越多的证据支持自噬在慢性淋巴细胞白血病中的作用；然而，到目前为止，还没有明确的线索。为了阐明这个问题，我们开展了一项初步研究，以测量慢性淋巴细胞白血病患者外周血单个核细胞中的自噬通量，并探讨其与经典临床/分析参数的相关性。
方法/患者：33 例慢性淋巴细胞白血病患者参与了这项研究。通过 Western blot 测定 p62 和脂化 LC3 蛋白的水平来确定外周血单个核细胞中的自噬通量。此外，通过 RT-qPCR 分析 p62 mRNA 水平。
结果
慢性淋巴细胞白血病患者的淋巴细胞增多和肿瘤性淋巴细胞百分比与自噬通量受阻呈统计学相关。
结论
自噬通量的改变可能在慢性淋巴细胞白血病的病理生理学中发挥重要作用。
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