Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418008, China.
The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Toxins (Basel). 2019 Jan 24;11(2):68. doi: 10.3390/toxins11020068.
() has been recently identified as a new species of spider in China. It lives in the same habitat as various other venomous spiders, including (), (), and (). The venom from these different species of spiders exhibits some similarities and some differences in terms of their biochemical and electrophysiological properties. With the objective to illustrate the diversity in venom peptide toxins and to establish the evolutionary relationship between different spider species, we first performed transcriptomic analysis on a cDNA library from the venom gland of . We identified 146 novel toxin-like sequences, which were classified into eighteen different superfamilies. This transcriptome was then compared with that of , which revealed that the putative toxins from both spider venoms may have originated from the same ancestor, although novel toxins evolved independently in the two species. A BLAST search and pharmacological analysis revealed that the two venoms have similar sodium channel modulation activity. This study provides insights into the venom of two closely related species of spider, which will prove useful towards understanding the structure and function of their toxins.
() 最近被鉴定为中国的一种新蜘蛛物种。它与各种其他有毒蜘蛛生活在相同的栖息地，包括 (), (), 和 (）。这些不同种类蜘蛛的毒液在生化和电生理特性方面表现出一些相似性和一些差异。为了说明毒液肽毒素的多样性，并建立不同蜘蛛物种之间的进化关系，我们首先对 () 的毒腺 cDNA 文库进行了转录组分析。我们鉴定出了 146 种新型毒素样序列，它们分为十八个不同的超家族。然后将这个转录组与 () 的转录组进行比较，结果表明，两种蜘蛛毒液中的假定毒素可能来自同一个祖先，尽管两种毒液中的新毒素是独立进化的。BLAST 搜索和药理学分析表明，两种毒液具有相似的钠通道调节活性。这项研究深入了解了两种密切相关的蜘蛛毒液，这将有助于理解它们毒素的结构和功能。
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