Department of Biochemistry and Biotechnology, CCE, State University of Londrina, PO BOX 6001, Londrina, PR, 86051-990, Brazil.
Appl Microbiol Biotechnol. 2019 Mar;103(5):2193-2203. doi: 10.1007/s00253-019-09646-w. Epub 2019 Jan 26.
The objective of this study was to develop biodegradable coatings for agriculture crop seeds based on starch, gelatin, and polyvinyl alcohol (PVA). Developed materials were characterized according to their microstructures, barrier properties, influence on germination of maize seeds, and ability to sustain Azospirillum brasilense Ab-V5 viability in coated maize seeds. The coatings were obtained employing different proportions of starch, gelatin, and PVA, ranging from 0 to 3.0 g/100 g of each material, respectively. Samples formulated with the pure polymers showed the highest values of water absorption capacity, solubility, and water vapor permeability, and the ternary mixtures showed the lowest values. Single polymer formulations and the binary starch-gelatin mixture (CS50GL50) favored maize seeds germination compared to the uncoated maize seeds. In addition, seed coating obtained from CS50GL50 formulation resulted in A. brasilense Ab-V5 viability in coated seeds up to 15 days after bacterial immobilization, being considered a promising low-cost, biodegradable, and renewable source material to be used in agriculture.
本研究旨在基于淀粉、明胶和聚乙烯醇(PVA)开发用于农业作物种子的可生物降解涂层。根据微观结构、阻隔性能、对玉米种子发芽的影响以及在包衣玉米种子中维持巴西固氮螺菌 Ab-V5 生存能力的能力对开发材料进行了表征。采用不同比例的淀粉、明胶和 PVA,分别为 0 至 3.0 g/100 g 材料,制备了涂层。由纯聚合物制备的样品表现出最高的吸水率、溶解度和水蒸气透过率,而三元混合物则表现出最低的值。与未包衣的玉米种子相比,单一聚合物配方和二元淀粉-明胶混合物(CS50GL50)有利于玉米种子发芽。此外,CS50GL50 配方获得的种子涂层在细菌固定化后 15 天内保持巴西固氮螺菌 Ab-V5 的活力,被认为是一种有前途的低成本、可生物降解和可再生的农业用源材料。