沉香精油对临床、食品和环境来源的多重耐药（MDR）微生物的抗菌活性。

Antimicrobial Activity of Agarwood Oil Against Multiple-Drug-Resistant (MDR) Microbes of Clinical, Food and Environmental Origin.

机构信息

Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, India.

Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, India.

出版信息

Curr Drug Discov Technol. 2020;17(3):348-356. doi: 10.2174/1570163816666190125163536.

DOI:10.2174/1570163816666190125163536

PMID:30686261

Abstract

BACKGROUND AND OBJECTIVES

Multiple-Drug-Resistance (MDR) among bacteria is an imminent problem and alternative therapies are seen as a future abode. Agarwood Oil (AO) is described to possess antimicrobial activity besides many other medicinal utilities. This paper discusses the antimicrobial activity of AO on MDR and non-MDR strains of microbes of 69 genera isolated from clinical and non-clinical samples.

METHODS AND RESULTS

In this study sensitivity of microbes was determined for conventional antimicrobials and AO using disc diffusion assay followed by determination of minimum inhibitory concentration (MIC) using agar well dilution assay. A total of 18.5% (522) strains were found sensitive to AO. Carbapenem resistant bacterial strains were more often (p, ≤0.01) resistant to antibiotics with 4.2 times more odds (99% CI, 2.99-5.90) of being MDR than carbapenem sensitive strains but no difference in their AO sensitivity was observed. However, MDR strains were more often (p, <0.001) resistant to AO than non-MDR strains. Bacteria isolated from dogs were more often sensitive to AO than those from buffaloes, human, horse, and cattle. On the other hand, bacteria from pigs were more often (p, ≤0.05) resistant to AO than bacteria from human, cattle, buffaloes, dogs, wild carnivores and birds. Oxidase positive Gram positive bacteria had 4.29 (95% CI, 2.94-6.27) times more odds to be AO sensitive than oxidase negative Gram negative bacteria. Bacillus species strains were the most sensitive bacteria to AO followed by strains of Streptococcus and Staphylococcus. The MIC of AO for different bacteria ranged from 0.01 mg/mL to > 2.56 mg/mL.

CONCLUSION

The study concluded that MDR and AO resistance had a similar trend and AO may not be seen as a good antimicrobial agent against MDR strains.

摘要

背景与目的

细菌的多药耐药性（MDR）是一个迫在眉睫的问题，替代疗法被视为未来的治疗方法。沉香木油（AO）除了具有许多其他药用功效外，还被描述具有抗菌活性。本文讨论了 AO 对从临床和非临床样本中分离出的 69 属 MDR 和非 MDR 微生物的抗菌活性。

方法和结果

在这项研究中，使用纸片扩散法测定了微生物对常规抗菌药物和 AO 的敏感性，然后使用琼脂孔稀释法测定最小抑菌浓度（MIC）。共有 18.5%（522 株）的菌株对 AO 敏感。碳青霉烯类耐药细菌菌株更常（p，≤0.01）对具有 4.2 倍更高的（99%CI，2.99-5.90）耐药几率的抗生素耐药，而与碳青霉烯类敏感菌株相比，它们对 AO 的敏感性没有差异。然而，MDR 菌株比非 MDR 菌株更常（p，<0.001）对 AO 耐药。从狗中分离的细菌比从水牛、人、马和牛中分离的细菌更常对 AO 敏感。另一方面，从猪中分离的细菌比从人、牛、水牛、狗、野生食肉动物和鸟类中分离的细菌更常（p，≤0.05）对 AO 耐药。氧化酶阳性革兰氏阳性细菌对 AO 敏感的几率是氧化酶阴性革兰氏阴性细菌的 4.29 倍（95%CI，2.94-6.27）。芽孢杆菌属菌株是对 AO 最敏感的细菌，其次是链球菌属和葡萄球菌属菌株。不同细菌的 AO MIC 值范围从 0.01mg/mL 到>2.56mg/mL。