Gbongue Louis-Raymond, Lalaymia Ismahen, Zeze Adolphe, Delvaux Bruno, Declerck Stéphane
Laboratoire de Biotechnologies Végétale et Microbienne, Unité Mixte de Recherche et d'Innovation en Sciences Agronomiques et Génie Rural, Institut National Polytechnique Felix Houphouët-Boigny, Yamoussoukro, Côte d'Ivoire.
Mycology, Applied Microbiology, Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
Front Plant Sci. 2019 Jan 9;9:1977. doi: 10.3389/fpls.2018.01977. eCollection 2018.
This work aimed to test the hypothesis that the combination of arbuscular mycorrhizal fungi (AMF) and accumulation of silicon (Si) in banana plants via its uptake and transport by the fungus reduces the incidence of Black Leaf Steak Disease (BLSD) caused by . A pot experiment was conducted to compare BLSD symptoms on leaves of banana plants colonized or not by the AMF MUCL 41833 and exposed or not to Si added to the growth substrate. A marked increase in plant growth parameters (i.e., pseudostem diameter and height, leaf surface area, shoot, root and total dry weight) as well as accumulation of Si, P, and Ca were noticed in the AMF-colonized banana plants in presence as well as in absence of Si added to the growth substrate. Similarly Si addition to the substrate increased plant growth parameters. Leave symptoms caused by the pathogen were observed in all the treatments but were reduced in presence of AMF as well as in presence of Si added to the growth substrate. The more drastic reduction was noticed in the AMF-colonized plants with Si added to the growth substrate. The Severity Index as well as Area Under Disease Progress Curve were considerably decreased both at 21 (∼48% and 48%, respectively) and 35 days (∼21% and ∼32%, respectively) after inoculation of the pathogen as compared with non-AMF-colonized plants in absence of Si added to the substrate. Our findings revealed that AMF-colonized banana plants grown in a subs-trate supplemented with Si were less impacted by than non-colonized plants grown without Si added to the growth substrate. The combination of AMF-colonized banana plants (during the weaning phase or ) with the application of Si to soil seems thus a thoughtful option to mitigate the impact of BLSD in bananas, although such strategy needs first to be evaluated under field conditions to appraise its real potential.
丛枝菌根真菌（AMF）与香蕉植株通过真菌吸收和运输积累硅（Si）相结合，可降低由……引起的黑叶条斑病（BLSD）的发病率。进行了一项盆栽试验，以比较接种或未接种AMF MUCL 41833且生长基质中添加或未添加Si的香蕉植株叶片上的BLSD症状。在生长基质中添加或未添加Si的情况下，接种AMF的香蕉植株的植物生长参数（即假茎直径和高度、叶面积、地上部、根部和总干重）以及Si、P和Ca的积累均显著增加。同样，向基质中添加Si也增加了植物生长参数。在所有处理中均观察到了由病原体引起的叶片症状，但在接种AMF以及生长基质中添加Si的情况下症状有所减轻。在生长基质中添加Si的接种AMF的植株中症状减轻更为明显。与未接种AMF且生长基质中未添加Si的植株相比，接种病原体后21天（分别约为48%和48%）和35天（分别约为21%和32%）时，病情严重指数以及病害进展曲线下面积均显著降低。我们的研究结果表明，在添加Si的基质中生长的接种AMF的香蕉植株比在生长基质中未添加Si的未接种植株受……的影响更小。因此，接种AMF的香蕉植株（在断奶期或……期间）与向土壤中施用Si相结合似乎是减轻香蕉中BLSD影响的一个明智选择，尽管这种策略首先需要在田间条件下进行评估以评估其实际潜力。
